[HTML][HTML] Integrative approaches for species delimitation in Ascomycota

SSN Maharachchikumbura, Y Chen, HA Ariyawansa… - Fungal Diversity, 2021 - Springer
Biodiversity loss from disturbances caused by human activities means that species are
disappearing at an ever increasing rate. The high number of species that have yet to be …

[PDF][PDF] A simple guide to de novo transcriptome assembly and annotation

V Raghavan, L Kraft, F Mesny… - Briefings in …, 2022 - academic.oup.com
A transcriptome constructed from short-read RNA sequencing (RNA-seq) is an easily
attainable proxy catalog of protein-coding genes when genome assembly is unnecessary …

[HTML][HTML] CheckM2: a rapid, scalable and accurate tool for assessing microbial genome quality using machine learning

A Chklovski, DH Parks, BJ Woodcroft, GW Tyson - Nature Methods, 2023 - nature.com
Advances in sequencing technologies and bioinformatics tools have dramatically increased
the recovery rate of microbial genomes from metagenomic data. Assessing the quality of …

The Chinese pine genome and methylome unveil key features of conifer evolution

S Niu, J Li, W Bo, W Yang, A Zuccolo, S Giacomello… - Cell, 2022 - cell.com
Conifers dominate the world's forest ecosystems and are the most widely planted tree
species. Their giant and complex genomes present great challenges for assembling a …

BRAKER2: automatic eukaryotic genome annotation with GeneMark-EP+ and AUGUSTUS supported by a protein database

T Brůna, KJ Hoff, A Lomsadze, M Stanke… - NAR genomics and …, 2021 - academic.oup.com
The task of eukaryotic genome annotation remains challenging. Only a few genomes could
serve as standards of annotation achieved through a tremendous investment of human …

Significantly improving the quality of genome assemblies through curation

K Howe, W Chow, J Collins, S Pelan, DL Pointon… - …, 2021 - academic.oup.com
Genome sequence assemblies provide the basis for our understanding of biology.
Generating error-free assemblies is therefore the ultimate, but sadly still unachieved goal of …

[HTML][HTML] A route to de novo domestication of wild allotetraploid rice

H Yu, T Lin, X Meng, H Du, J Zhang, G Liu, M Chen… - Cell, 2021 - cell.com
Cultivated rice varieties are all diploid, and polyploidization of rice has long been desired
because of its advantages in genome buffering, vigorousness, and environmental …

Wildfire-dependent changes in soil microbiome diversity and function

AR Nelson, AB Narrowe, CC Rhoades, TS Fegel… - Nature …, 2022 - nature.com
Forest soil microbiomes have crucial roles in carbon storage, biogeochemical cycling and
rhizosphere processes. Wildfire season length, and the frequency and size of severe fires …

[HTML][HTML] Genome assembly and genetic dissection of a prominent drought-resistant maize germplasm

T Tian, S Wang, S Yang, Z Yang, S Liu, Y Wang… - Nature Genetics, 2023 - nature.com
In the context of climate change, drought is one of the most limiting factors that influence
crop production. Maize, as a major crop, is highly vulnerable to water deficit, which causes …

Widespread introgression across a phylogeny of 155 Drosophila genomes

A Suvorov, BY Kim, J Wang, EE Armstrong, D Peede… - Current Biology, 2022 - cell.com
Genome-scale sequence data have invigorated the study of hybridization and introgression,
particularly in animals. However, outside of a few notable cases, we lack systematic tests for …