Many bacterial cellular processes interact intimately with the chromosome. Such interplay is
the major driving force of genome structure or organization. Interactions take place at …

We present a new physical biology approach to understanding the relationship between the
organization and segregation of bacterial chromosomes. We posit that replicated …

Replicating bacterial chromosomes continuously demix from each other and segregate
within a compact volume inside the cell called the nucleoid. Although many proteins …

Recent experiments carried out in the dense cytoplasm of living cells have highlighted the
importance of proteome composition and nonspecific intermolecular interactions in …

Atomic force microscopy has proven to be a valuable technique to characterize the
mechanical and morphological properties of heterogeneous soft materials such as …

In Escherichia coli, ribosomes concentrate near the cylindrical wall and at the endcaps,
whereas the chromosomal DNA segregates in the more centrally located nucleoid. A simple …

How confinement or a physical constraint modifies polymer chains is not only a classical
problem in polymer physics but also relevant in a variety of contexts such as single-molecule …

Chromosomes in living cells are strongly confined but show a high level of spatial
organization. Similarly, confined polymers display intriguing organizational and …

The Escherichia coli chromosome is a circular DNA molecule that is∼ 1000 times
compacted in the living cell, where it occupies∼ 15% of the cellular volume. The genome is …

What physical mechanism leads to organization of a highly condensed and confined circular
chromosome? Computational modeling shows that confinement-induced organization is …