Field-usable lateral flow immunoassay for the rapid detection of a macluravirus, large cardamom chirke virus

Y Maheshwari, S Vijayanandraj, RK Jain… - Journal of Virological …, 2018 - Elsevier
A simple and rapid lateral flow immunoassay (LFIA) was developed by utilizing gold
nanoparticles conjugated to a polyclonal antibody against coat protein of large cardamom …

[HTML][HTML] A simplified multiplex PCR assay for simultaneous detection of six viruses infecting diverse chilli species in India and its application in field diagnosis

OP Devi, SK Sharma, K Sanatombi, KS Devi, N Pathaw… - Pathogens, 2022 - mdpi.com
Chilli is infected by at least 65 viruses globally, with a mixed infection of multiple viruses
leading to severe losses being a common occurrence. A simple diagnostic procedure that …

On-site detection of Citrus tristeza virus (CTV) by lateral flow immunoassay using polyclonal antisera derived from virions produced by a recombinant CTV

Y Maheshwari, V Selvaraj, S Hajeri, C Ramadugu… - Phytoparasitica, 2017 - Springer
Maintenance of virus-free citrus in nurseries and orchards is essential to control the spread
of aphid-transmitted Citrus tristeza virus (CTV). To this end, a lateral flow immunoassay …

Development of reverse transcriptase-recombinase polymerase amplification (RT-RPA) assay for rapid detection of large cardamom chirke virus

P Malavika, AI Bhat, M Greeshma - VirusDisease, 2024 - Springer
Large cardamom chirke virus (LCCV) causing chirke disease of large cardamom is a major
production constraint of this crop. Rapid and accurate detection of LCCV is important for …

Highly Efficient Immunodiagnosis of Episomal Banana streak MY virus Using Polyclonal Antibodies Raised Against Recombinant Viral‐Associated Protein

R Selvarajan, V Balasubramanian… - Journal of …, 2016 - Wiley Online Library
B anana streak MY virus (BSMYV) is the causal agent of viral leaf streak disease of banana,
which leads to considerable losses in banana production in most of the banana‐growing …

Efficient immunodiagnosis of Citrus yellow mosaic virus using polyclonal antibodies with an expressed recombinant virion-associated protein

PV Kumar, SK Sharma, N Rishi, VK Baranwal - 3 Biotech, 2018 - Springer
Citrus yellow mosaic virus (CYMV) is a member of genus Badnavirus of the family
Caulimoviridae. It is the causal agent of citrus yellow mosaic disease in citrus and causes …

[PDF][PDF] Morphological and molecular identification of major fungal disease of large cardamom (Amomum subulatum Roxb.) in Sikkim and its management using …

K Gurung - 2020 - dspace.cus.ac.in
Sikkim is a tiny beautiful hill state lying in the lap of the Eastern Himalaya with lash green
vegetations, high mountains, glaciers, rivers and streams. Nature is generous to the region …

Next-generation molecular diagnostics for plant viruses

A Srivastava, S Srivastava, T Gupta, N Rishi - Virus Diseases of …, 2021 - Springer
Viral disease is a major constraint for the yield loss in many economically important crops
and ultimately causes economic losses. Plant viruses are distributed from one infected crop …

Engineered antibody fragments for immunodiagnosis of papaya ringspot virus

Y Maheshwari, HN Verma, RK Jain, B Mandal - Molecular biotechnology, 2015 - Springer
The present study was undertaken to clone and express the genes encoding antibody to the
recombinant coat protein (rCP) of Papaya ringspot virus (PRSV) and to assess the …

[PDF][PDF] First report of large cardamom chirke virus, a macluravirus naturally infecting chili crop in India

SK Sharma, NT Chanu, YR Anand, YH Singh, S Singh… - Plant Dis, 2019 - researchgate.net
Chilli (Capsicum spp.) is an economically important spice crop widely grown in India. The
Sikkim-Darjeeling hills region of North East India is known for biodiversity of chilli landraces …