Control of asparagine homeostasis in Bacillus subtilis: identification of promiscuous amino acid importers and exporters

J Meißner, M Königshof, K Wrede… - Journal of …, 2024 - Am Soc Microbiol
The Gram-positive model bacterium B. subtilis is able to import all proteinogenic amino
acids from the environment as well as to synthesize them. However, the players involved in …

Visualizing dynamic competence pili and DNA capture throughout the long axis of Bacillus subtilis

JD Zuke, R Erickson, KR Hummels… - Journal of …, 2023 - Am Soc Microbiol
The first step in the process of bacterial natural transformation is DNA capture. Although long
hypothesized based on genetics and functional experiments, the pilus structure responsible …

Knockout of ykcB, a Putative Glycosyltransferase, Leads to Reduced Susceptibility to Vancomycin in Bacillus subtilis

K Ishikawa, R Shirakawa, D Takano… - Journal of …, 2022 - Am Soc Microbiol
Vancomycin resistance of Gram-positive bacteria poses a serious health concern around the
world. In this study, we searched for vancomycin-tolerant mutants from a gene deletion …

Poly-gamma-glutamic acid secretion protects Bacillus subtilis from zinc and copper intoxication

R Deol, A Louis, HL Glazer, W Hosseinion… - Microbiology …, 2022 - Am Soc Microbiol
Zinc and copper are essential micronutrients that serve as a cofactors for numerous
enzymes. However, when present at elevated concentrations, zinc and copper are highly …

A high-content microscopy screening identifies new genes involved in cell width control in Bacillus subtilis

D Juillot, C Cornilleau, N Deboosere, C Billaudeau… - Msystems, 2021 - Am Soc Microbiol
How cells control their shape and size is a fundamental question of biology. In most bacteria,
cell shape is imposed by the peptidoglycan (PG) polymeric meshwork that surrounds the …

RqcH supports survival in the absence of non-stop ribosome rescue factors

K Callan, CR Prince, HA Feaga - bioRxiv, 2024 - biorxiv.org
Ribosomes frequently translate truncated or damaged mRNAs due to the extremely short
half-life of mRNAs in bacteria. When ribosomes translate mRNA that lacks a stop codon (non …