Macromolecular crowding is more than hard-core repulsions
Cells are crowded, but proteins are almost always studied in dilute aqueous buffer. We
review the experimental evidence that crowding affects the equilibrium thermodynamics of …
review the experimental evidence that crowding affects the equilibrium thermodynamics of …
Influence of nonspecific interactions on protein associations: implications for biochemistry in vivo
The cellular interior is composed of a variety of microenvironments defined by distinct local
compositions and composition-dependent intermolecular interactions. We review the …
compositions and composition-dependent intermolecular interactions. We review the …
Biomolecular interactions modulate macromolecular structure and dynamics in atomistic model of a bacterial cytoplasm
Biological macromolecules function in highly crowded cellular environments. The structure
and dynamics of proteins and nucleic acids are well characterized in vitro, but in vivo …
and dynamics of proteins and nucleic acids are well characterized in vitro, but in vivo …
Dynamic cluster formation determines viscosity and diffusion in dense protein solutions
We develop a detailed description of protein translational and rotational diffusion in
concentrated solution on the basis of all-atom molecular dynamics simulations in explicit …
concentrated solution on the basis of all-atom molecular dynamics simulations in explicit …
Depletion interactions modulate the binding between disordered proteins in crowded environments
Intrinsically disordered proteins (IDPs) abound in cellular regulation. Their interactions are
often transitory and highly sensitive to salt concentration and posttranslational modifications …
often transitory and highly sensitive to salt concentration and posttranslational modifications …
Single-molecule spectroscopy reveals polymer effects of disordered proteins in crowded environments
It is currently unclear how the crowded cellular milieu affects the structural distributions of
intrinsically disordered proteins (IDPs). Here we employ single-molecule Förster resonance …
intrinsically disordered proteins (IDPs). Here we employ single-molecule Förster resonance …
Crowding in cellular environments at an atomistic level from computer simulations
The effects of crowding in biological environments on biomolecular structure, dynamics, and
function remain not well understood. Computer simulations of atomistic models of …
function remain not well understood. Computer simulations of atomistic models of …
Resolving the enthalpy of protein stabilization by macromolecular crowding
Proteins in the cellular milieu reside in environments crowded by macromolecules and other
solutes. Although crowding can significantly impact the protein folded state stability, most …
solutes. Although crowding can significantly impact the protein folded state stability, most …
Excluded‐volume effects in living cells
Biomolecules evolve and function in densely crowded and highly heterogeneous cellular
environments. Such conditions are often mimicked in the test tube by the addition of artificial …
environments. Such conditions are often mimicked in the test tube by the addition of artificial …
Is the depletion force entropic? Molecular crowding beyond steric interactions
Cosolutes excluded from macromolecules create effective attractions between the excluding
macromolecules, and promote their self-association. This “depletion force” serves an …
macromolecules, and promote their self-association. This “depletion force” serves an …