Quantum chemical studies of mechanisms for metalloenzymes
Two decades ago, theoretical model calculations played a truly minor role in understanding
mechanisms of redox-active metalloenzymes. The methods available to treat transition …
mechanisms of redox-active metalloenzymes. The methods available to treat transition …
Nucleases: diversity of structure, function and mechanism
W Yang - Quarterly reviews of biophysics, 2011 - cambridge.org
Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo,
DNase or RNase, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes. In …
DNase or RNase, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes. In …
Reaction Mechanisms in Carbohydrate-Active Enzymes: Glycoside Hydrolases and Glycosyltransferases. Insights from ab Initio Quantum Mechanics/Molecular …
A Ardèvol, C Rovira - Journal of the American Chemical Society, 2015 - ACS Publications
Carbohydrate-active enzymes such as glycoside hydrolases (GHs) and glycosyltransferases
(GTs) are of growing importance as drug targets. The development of efficient competitive …
(GTs) are of growing importance as drug targets. The development of efficient competitive …
Early steps in the DNA base excision/single-strand interruption repair pathway in mammalian cells
Base excision repair (BER) is an evolutionarily conserved process for maintaining genomic
integrity by eliminating several dozen damaged (oxidized or alkylated) or inappropriate …
integrity by eliminating several dozen damaged (oxidized or alkylated) or inappropriate …
Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics
CRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …
Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is …
[HTML][HTML] Human flap endonuclease structures, DNA double-base flipping, and a unified understanding of the FEN1 superfamily
SE Tsutakawa, S Classen, BR Chapados, AS Arvai… - Cell, 2011 - cell.com
Summary Flap endonuclease (FEN1), essential for DNA replication and repair, removes
RNA and DNA 5′ flaps. FEN1 5′ nuclease superfamily members acting in nucleotide …
RNA and DNA 5′ flaps. FEN1 5′ nuclease superfamily members acting in nucleotide …
[HTML][HTML] Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced …
S Classen, GL Hura, JM Holton, RP Rambo… - Journal of applied …, 2013 - scripts.iucr.org
The SIBYLS beamline (12.3. 1) of the Advanced Light Source at Lawrence Berkeley
National Laboratory, supported by the US Department of Energy and the National Institutes …
National Laboratory, supported by the US Department of Energy and the National Institutes …
Monovalent metal ion binding promotes the first transesterification reaction in the spliceosome
Cleavage and formation of phosphodiester bonds in nucleic acids is accomplished by large
cellular machineries composed of both protein and RNA. Long thought to rely on a two …
cellular machineries composed of both protein and RNA. Long thought to rely on a two …
Catalytic metal ions and enzymatic processing of DNA and RNA
Conspectus Two-metal-ion-dependent nucleases cleave the phosphodiester bonds of
nucleic acids via the two-metal-ion (2M) mechanism. Several high-resolution X-ray …
nucleic acids via the two-metal-ion (2M) mechanism. Several high-resolution X-ray …
The DNA Exonucleases of Escherichia coli
ST Lovett - EcoSal Plus, 2011 - Am Soc Microbiol
DNA exonucleases, enzymes that hydrolyze phosphodiester bonds in DNA from a free end,
play important cellular roles in DNA repair, genetic recombination and mutation avoidance …
play important cellular roles in DNA repair, genetic recombination and mutation avoidance …