Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

MEMOTE for standardized genome-scale metabolic model testing

C Lieven, ME Beber, BG Olivier, FT Bergmann… - Nature …, 2020 - nature.com
To the Editor—Reconstructing metabolic reaction networks enables the development of
testable hypotheses of an organism's metabolism under different conditions 1. State-of-the …

A systematic assessment of current genome-scale metabolic reconstruction tools

SN Mendoza, BG Olivier, D Molenaar, B Teusink - Genome biology, 2019 - Springer
Background Several genome-scale metabolic reconstruction software platforms have been
developed and are being continuously updated. These tools have been widely applied to …

High‐quality genome‐scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities

J Nogales, J Mueller, S Gudmundsson… - Environmental …, 2020 - Wiley Online Library
Genome‐scale reconstructions of metabolism are computational species‐specific
knowledge bases able to compute systemic metabolic properties. We present a …

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

[HTML][HTML] Biobased PET from lignin using an engineered cis, cis-muconate-producing Pseudomonas putida strain with superior robustness, energy and redox …

M Kohlstedt, A Weimer, F Weiland, J Stolzenberger… - Metabolic …, 2022 - Elsevier
Polyethylene terephthalate (PET), the most common synthetic polyester today, is largely
produced from fossil resources, contributing to global warming. Consequently, sustainable …

[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida

M Martin-Pascual, C Batianis, L Bruinsma… - Biotechnology …, 2021 - Elsevier
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …

A genome-scale metabolic model of Saccharomyces cerevisiae that integrates expression constraints and reaction thermodynamics

O Oftadeh, P Salvy, M Masid, M Curvat… - Nature …, 2021 - nature.com
Eukaryotic organisms play an important role in industrial biotechnology, from the production
of fuels and commodity chemicals to therapeutic proteins. To optimize these industrial …

[HTML][HTML] GC-MS-based 13C metabolic flux analysis resolves the parallel and cyclic glucose metabolism of Pseudomonas putida KT2440 and Pseudomonas …

M Kohlstedt, C Wittmann - Metabolic engineering, 2019 - Elsevier
The genus Pseudomonas comprises approximately 200 species with numerous isolates that
are common inhabitants of soil, water, and vegetation and has been of particular interest for …