Plant genome editing: ever more precise and wide reaching

S Sukegawa, H Saika, S Toki - The Plant Journal, 2021 - Wiley Online Library
Genome‐editing technologies consisting of targeted mutagenesis and gene targeting
enable us to modify genes of interest rapidly and precisely. The discovery in 2012 of …

Building Blocks of Artificial CRISPR-Based Systems beyond Nucleases

AA Kuzmin, AN Tomilin - International Journal of Molecular Sciences, 2022 - mdpi.com
Tools developed in the fields of genome engineering, precise gene regulation, and synthetic
gene networks have an increasing number of applications. When shared with the scientific …

SUPPRESSOR OF GAMMA RESPONSE 1 plays rice-specific roles in DNA damage response and repair

A Nishizawa-Yokoi, R Motoyama, T Tanaka… - Plant …, 2023 - academic.oup.com
Land plants are constantly exposed to environmental stresses and have developed
complicated defense systems, including DNA damage response (DDR) and DNA repair …

Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis

Z Zhang, W Zeng, W Zhang, J Li, D Kong… - Plant …, 2022 - academic.oup.com
Homologous recombination-mediated gene targeting (GT) enables precise sequence
knockin or sequence replacement, and thus is a powerful tool for heritable precision …

Precise and heritable gene targeting in rice using a sequential transformation strategy

W Zhang, R Wang, D Kong, F Peng, M Chen… - Cell Reports …, 2023 - cell.com
Gene targeting (GT) is a powerful tool for modifying endogenous genomic sequences of
interest, such as sequence replacement and gene knockin. Although the efficiency of GT is …

[HTML][HTML] Genome editing in plants

N Wada, K Osakabe, Y Osakabe - Gene and Genome Editing, 2022 - Elsevier
Genome editing technologies have brought dramatic changes in many fields of research,
including plant sciences. Zinc finger nuclease, transcription activator-like effector nuclease …

Plant genome engineering from lab to field—a Keystone Symposia report

J Cable, PC Ronald, D Voytas, F Zhang… - Annals of the New …, 2021 - Wiley Online Library
Facing the challenges of the world's food sources posed by a growing global population and
a warming climate will require improvements in plant breeding and technology. Enhancing …

Precise genome editing in miRNA target site via gene targeting and subsequent single-strand-annealing-mediated excision of the marker gene in plants

N Ohtsuki, K Kizawa, A Mori… - Frontiers in Genome …, 2021 - frontiersin.org
Gene targeting (GT) enables precise genome modification—eg, the introduction of base
substitutions—using donor DNA as a template. Combined with clean excision of the …

Real-time monitoring of key gene products involved in rice photoperiodic flowering

H Yoshioka, K Kimura, Y Ogo, N Ohtsuki… - Frontiers in Plant …, 2021 - frontiersin.org
Flowering is an important biological process through which plants determine the timing of
reproduction. In rice, florigen mRNA is induced more strongly when the day length is shorter …

Genome editing technology and its application to metabolic engineering in rice

S Sukegawa, S Toki, H Saika - Rice, 2022 - Springer
Genome editing technology can be used for gene engineering in many organisms. A target
metabolite can be fortified by the knockout and modification of target genes encoding …