The miniature CRISPR-Cas12m effector binds DNA to block transcription
CRISPR-Cas are prokaryotic adaptive immune systems. Cas nucleases generally use
CRISPR-derived RNA guides to specifically bind and cleave DNA or RNA targets. Here, we …
CRISPR-derived RNA guides to specifically bind and cleave DNA or RNA targets. Here, we …
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type VA CRISPR-Cas anti-
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …
Accelerated CRISPR/Cas12a-based small molecule detection using bivalent aptamer
X Li, X Chen, M Mao, C Peng, Z Wang - Biosensors and Bioelectronics, 2022 - Elsevier
CRISPR/Cas holds great promise for biosensing applications, however, restricted to nucleic
acid targets. Here, we broaden the sensing target of CRISPR/Cas to small molecules via …
acid targets. Here, we broaden the sensing target of CRISPR/Cas to small molecules via …
HLTF disrupts Cas9-DNA post-cleavage complexes to allow DNA break processing
G Reginato, MR Dello Stritto, Y Wang, J Hao… - Nature …, 2024 - nature.com
The outcome of CRISPR-Cas-mediated genome modifications is dependent on DNA double-
strand break (DSB) processing and repair pathway choice. Homology-directed repair (HDR) …
strand break (DSB) processing and repair pathway choice. Homology-directed repair (HDR) …
An alpha-helical lid guides the target DNA toward catalysis in CRISPR-Cas12a
CRISPR-Cas12a is a powerful RNA-guided genome-editing system that generates double-
strand DNA breaks using its single RuvC nuclease domain by a sequential mechanism in …
strand DNA breaks using its single RuvC nuclease domain by a sequential mechanism in …
Physiology of γ-aminobutyric acid production by Akkermansia muciniphila
P Konstanti, K Ligthart, C Fryganas… - Applied and …, 2024 - Am Soc Microbiol
Gut bacteria hold the potential to produce a broad range of metabolites that can modulate
human functions, including molecules with neuroactive potential. One such molecule is γ …
human functions, including molecules with neuroactive potential. One such molecule is γ …
Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters
The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI
systems has opened a new era in the field of nucleic acid-based detection. Here, we report …
systems has opened a new era in the field of nucleic acid-based detection. Here, we report …
Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA
CRISPR-Cas9 systems are enriched in human pathogenic bacteria and have been linked to
cytotoxicity by an unknown mechanism. Here, we show that upon infection of human cells …
cytotoxicity by an unknown mechanism. Here, we show that upon infection of human cells …
Cas12a-based direct visualization of nanoparticle-stabilized fluorescence signal for multiplex detection of DNA methylation biomarkers
Y Li, M Cai, W Zhang, Y Liu, X Yuan, N Han, J Li… - Biosensors and …, 2024 - Elsevier
Abstract The CRISPR-Cas12a RNA-guided complexes hold immense promise for nucleic
acid detection. However, limitations arise from their specificity in detecting off-targets and the …
acid detection. However, limitations arise from their specificity in detecting off-targets and the …
Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants
Cas9 is an RNA-guided endonuclease in the bacterial CRISPR–Cas immune system and a
popular tool for genome editing. The commonly used Streptococcus pyogenes Cas9 …
popular tool for genome editing. The commonly used Streptococcus pyogenes Cas9 …