P450BM3 (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been
extensively studied over a period of almost forty years. The enzyme has been redesigned to …

Cytochromes P450 (CYPs) belong to the superfamily of heme b containing
monooxygenases with currently more than 21,000 members. These enzymes accept a vast …

Butanol, an important chemical feedstock and advanced biofuel, is produced by Clostridium
species. Various efforts have been made to transfer the clostridial 1-butanol pathway into …

Oxidoreductase is the largest class of enzymes and has broad applications in biotechnology
since a number of bioconversions involve oxidation/reduction reactions. Coenzymes are …

Asymmetric catalysis plays a key role in modern synthetic organic chemistry, with synthetic
catalysts and enzymes being the two available options. During the latter part of the last …

The widespread adoption of biocatalysis by industry to perform highly selective chemical
syntheses has been made possible only by the development of highly effective methods in …

Engineering the cofactor availability is a common strategy of metabolic engineering to
improve the production of many industrially important compounds. In this work, a de novo …

Fossil fuels (crude oil, coal, and natural gas) represent the current primary source of the
world's energy supply, accounting for 87% of global energy. 1 World energy consumption is …

The efficient regeneration of cofactors is vital for the establishment of biocatalytic processes.
Formate is an ideal electron donor for cofactor regeneration due to its general availability …

Random mutagenesis is a powerful tool for generating enzymes, proteins, entire metabolic
pathways, or even entire genomes with desired or improved properties. This technology is …