Agnostic sequencing for detection of viral pathogens

NPG Gauthier, SD Chorlton, M Krajden… - Clinical microbiology …, 2023 - Am Soc Microbiol
The advent of next-generation sequencing (NGS) technologies has expanded our ability to
detect and analyze microbial genomes and has yielded novel molecular approaches for …

Research on a Magnetic Separation-Based Rapid Nucleic Acid Extraction System and Its Detection Applications

Y Li, S Liu, Y Wang, Y Wang, S Li, N He, Y Deng… - Biosensors, 2023 - mdpi.com
Nucleic acid extraction represents the “first step” in molecular diagnostic experiments. The
quality of this extraction serves as a fundamental prerequisite for ensuring the accuracy of …

Validation of an automated, end-to-end metagenomic sequencing assay for agnostic detection of respiratory viruses

NPG Gauthier, W Chan, K Locher… - The Journal of …, 2024 - academic.oup.com
Background Current molecular diagnostics are limited in the number and type of detectable
pathogens. Metagenomic next-generation sequencing (mNGS) is an emerging, and …

Importance of sample input volume for accurate SARS-CoV-2 qPCR testing

Y He, T Xie, Q Tu, Y Tong - Analytica Chimica Acta, 2022 - Elsevier
Nucleic acid testing is the most widely used detection method for severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease …

[HTML][HTML] Developing novel liquid biopsy by selective capture of viral RNA on magnetic beads to detect COVID-19

MA Kerachian, SA Jamehdar, M Azghandi… - Iranian journal of …, 2022 - ncbi.nlm.nih.gov
Objective (s): Early, specific, and sensitive detection methods of COVID-19 are essential for
force stopping its worldwide infection. Although CT images of the lung and/or viral RNA …

Extraction Bottleneck in the Diagnosis of SARS-CoV-2: Evaluation of an Alternative Protocol Derived from Veterinary Use

P Bottino, E Zanotto, F Sidoti, L Pastrone, R Piva… - Microorganisms, 2023 - mdpi.com
The COVID-19 pandemic represented a challenge for health-care systems, and a major
bottleneck in SARS-CoV-2 diagnosis was the unavailability of extraction reagents. To …

[PDF][PDF] 低拷贝病毒RNA 捕获及多重实时荧光定量PCR 检测

余佳, 李晓宁, 肖君华, 李凯, 周宇荀 - 现代生物医学进展, 2022 - biomed.cnjournals.com
摘要目的: 为提高COVID-19 检测灵敏度, 减少新冠患者临床假阴性检测结果, 本研究建立了一种
SARS-CoV-2 低拷贝假病毒RNA 捕获方法. 方法: 利用链霉亲和素磁珠, 设计并合成生物素探针 …

Development of Magnetic-Silica Particles and In-house Buffers Kit for SARS-CoV-2 and CDV RNA Extraction

AD Prasetya, M Muflikhah, WZ Lubis… - Indonesian Journal of …, 2024 - journal.ugm.ac.id
Since the end of 2019, COVID-19 pandemic caused by the novel SARS-CoV-2 has become
a serious problem for the world. Accurate and rapid techniques in testing and tracing are …

Applications of metagenomic sequencing for virus detection and characterization of upper respiratory infections

N Gauthier - 2024 - open.library.ubc.ca
The application of next-generation sequencing technologies to answer more routine
diagnostic and applied microbiological questions is becoming increasingly feasible. Current …

[PDF][PDF] MAJOR ARTICLE

NPG Gauthier, W Chan, K Locher, D Smailus, R Coope… - 2024 - scholar.archive.org
Background: Current molecular diagnostics are limited in the number and type of detectable
pathogens. Metagenomic next generation sequencing (mNGS) is an emerging, and …