Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution

G Janusz, A Pawlik, J Sulej… - FEMS microbiology …, 2017 - academic.oup.com
Extensive research efforts have been dedicated to describing degradation of wood, which is
a complex process; hence, microorganisms have evolved different enzymatic and non …

Lignin valorisation via enzymes: A sustainable approach

RR Singhania, AK Patel, T Raj, CW Chen… - Fuel, 2022 - Elsevier
Lignin is the 3rd most abundant biopolymer surpassed by cellulose and hemicellulose and
is the most abundant aromatics resource available on earth for utilization by mankind. It was …

Same substrate, many reactions: oxygen activation in flavoenzymes

E Romero, JR Gómez Castellanos, G Gadda… - Chemical …, 2018 - ACS Publications
Over time, organisms have evolved strategies to cope with the abundance of dioxygen on
Earth. Oxygen-utilizing enzymes tightly control the reactions involving O2 mostly by …

The enigmatic reaction of flavins with oxygen

P Chaiyen, MW Fraaije, A Mattevi - Trends in biochemical sciences, 2012 - cell.com
The reaction of flavoenzymes with oxygen remains a fascinating area of research because
of its relevance for reactive oxygen species (ROS) generation. Several exciting recent …

The substrate oxidation mechanism of pyranose 2‐oxidase and other related enzymes in the glucose–methanol–choline superfamily

T Wongnate, P Chaiyen - The FEBS Journal, 2013 - Wiley Online Library
Enzymes in the glucose–methanol–choline (GMC) oxidoreductase superfamily catalyze the
oxidation of an alcohol moiety to the corresponding aldehyde. In this review, the current …

A survey of genes encoding H2O2-producing GMC oxidoreductases in 10 Polyporales genomes

P Ferreira, J Carro, A Serrano, AT Martínez - Mycologia, 2015 - Taylor & Francis
The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora
and a member of the Ganoderma lucidum complex) recently were sequenced to expand our …

Proton-coupled electron transfer and adduct configuration are important for C4a-hydroperoxyflavin formation and stabilization in a flavoenzyme

T Wongnate, P Surawatanawong… - Journal of the …, 2014 - ACS Publications
Determination of the mechanism of dioxygen activation by flavoenzymes remains one of the
most challenging problems in flavoenzymology for which the underlying theoretical basis is …

Stabilization of C4a-hydroperoxyflavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms

K Thotsaporn, P Chenprakhon, J Sucharitakul… - Journal of Biological …, 2011 - ASBMB
p-Hydroxyphenylacetate (HPA) 3-hydroxylase is a two-component flavin-dependent
monooxygenase. Based on the crystal structure of the oxygenase component (C 2), His-396 …

One‐Pot Bioconversion of l‐Arabinose to l‐Ribulose in an Enzymatic Cascade

L Chuaboon, T Wongnate, P Punthong… - Angewandte Chemie …, 2019 - Wiley Online Library
This work reports the one‐pot enzymatic cascade that completely converts l‐arabinose to l‐
ribulose using four reactions catalyzed by pyranose 2‐oxidase (P2O), xylose reductase …

The 1.6 Å Crystal Structure of Pyranose Dehydrogenase from Agaricus meleagris Rationalizes Substrate Specificity and Reveals a Flavin Intermediate

TC Tan, O Spadiut, T Wongnate, J Sucharitakul… - PLoS …, 2013 - journals.plos.org
Pyranose dehydrogenases (PDHs) are extracellular flavin-dependent oxidoreductases
secreted by litter-decomposing fungi with a role in natural recycling of plant matter. All major …