CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Reversing the central dogma: RNA-guided control of DNA in epigenetics and genome editing
Summary The Central Dogma of the flow of genetic information is arguably the crowning
achievement of 20 th century molecular biology. Reversing the flow of information from RNA …
achievement of 20 th century molecular biology. Reversing the flow of information from RNA …
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts
Transcriptional effectors are protein domains known to activate or repress gene expression;
however, a systematic understanding of which effector domains regulate transcription across …
however, a systematic understanding of which effector domains regulate transcription across …
CRISPR-assisted transcription activation by phase-separation proteins
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has
been widely used for genome engineering and transcriptional regulation in many different …
been widely used for genome engineering and transcriptional regulation in many different …
On RNA-programmable gene modulation as a versatile set of principles targeting muscular dystrophies
S Capelletti, SCG Soto, MAFV Gonçalves - Molecular Therapy, 2024 - cell.com
The repurposing of RNA-programmable CRISPR systems from genome editing into
epigenome editing tools is gaining pace, including in research and development efforts …
epigenome editing tools is gaining pace, including in research and development efforts …
A modular dCas9-based recruitment platform for combinatorial epigenome editing
Targeted epigenome editing tools allow precise manipulation and investigation of genome
modifications, however they often display high context dependency and variable efficacy …
modifications, however they often display high context dependency and variable efficacy …
Artificial transcription factors: technology development and applications in cell reprogramming, genetic screen, and disease treatment
Y Sang, L Xu, Z Bao - Molecular Therapy, 2024 - cell.com
Gene dysregulations are associated with many human diseases, such as cancers and
hereditary diseases. Artificial transcription factors (ATFs) are synthetic molecular tools to …
hereditary diseases. Artificial transcription factors (ATFs) are synthetic molecular tools to …
Tailoring a CRISPR/Cas-based Epigenome Editor for Programmable Chromatin Acylation and Decreased Cytotoxicity
Engineering histone acylation states can inform mechanistic epigenetics and catalyze
therapeutic epigenome editing opportunities. Here, we developed engineered lysine …
therapeutic epigenome editing opportunities. Here, we developed engineered lysine …
Bidirectional epigenetic editing reveals hierarchies in gene regulation
CRISPR perturbation methods are limited in their ability to study non-coding elements and
genetic interactions. In this study, we developed a system for bidirectional epigenetic editing …
genetic interactions. In this study, we developed a system for bidirectional epigenetic editing …
Characterization of transcriptional enhancers in the chicken genome using CRISPR-mediated activation
DNA regulatory elements intricately control when, where, and how genes are activated.
Therefore, understanding the function of these elements could unveil the complexity of the …
Therefore, understanding the function of these elements could unveil the complexity of the …