When a virus infects a host cell, it hijacks the biosynthetic capacity of the cell to produce
virus progeny, a process that may take less than an hour or more than a week. The overall …

The ability to use double-stranded RNA to inhibit gene expression sequence-specifically
(RNA interference, or RNAi) is currently revolutionizing science and medicine alike …

Double-stranded RNA can induce gene silencing via a process known as RNA interference
(RNAi). Previously, we have shown that stable expression of a single shRNA targeting the …

Human immunodeficiency virus type 1 (HIV-1) replication in T cells can be inhibited by RNA
interference (RNAi) through short hairpin RNA (shRNA) expression from a lentiviral vector …

Arthropods harbor a diverse array of microbes that profoundly influence many aspects of
host biology, including vector competence. Additionally, symbionts can be engineered to …

Many microRNAs (miRNAs) are encoded within the introns of RNA Pol II transcripts, often as
polycistronic precursors. Here, we demonstrate the optimization of an intron encoding three …

RNA-mediated interference (RNAi) is a recently discovered process by which dsRNA is able
to silence specific gene functions. Although initially described in plants, nematodes and …

The field of directed RNA interference (RNAi) has rapidly developed into a highly promising
approach for specifically downregulating genes to alleviate disease pathology. This …

Chronic hepatitis C virus (HCV) infection has a major medical impact and current treatments
are often unsuccessful. RNA interference represents a promising new approach to tackling …

Recently discovered Clustered Regularly Interspaced Short Palindromic Repeats
(CRISPR)/Cas13 proteins are programmable RNA-guided ribonucleases that target single …