Escherichia coli expression system continues to dominate the bacterial expression systems
and remain to be the preferred system for laboratory investigations and initial development …

After decades of slow progress, the pace of research on membrane protein structures is
beginning to quicken thanks to various improvements in technology, including protein …

Background Membrane proteins are the targets of 50% of drugs, although they only
represent 1% of total cellular proteins. The first major bottleneck on the route to their …

Despite many high-profile successes, recombinant membrane protein production remains a
technical challenge; it is still the case that many fewer membrane protein structures have …

Roughly one-third of all proteins reside in biological membranes [1, 2]. Integral membrane
proteins (IMPs), which can only be released from the membrane by disruption of the …

Escherichia coli is by far the most widely used bacterial host for the production of membrane
proteins. Usually, different strains, culture conditions and production regimes are screened …

We developed a generic method for high-throughput cloning in bacteria that are less
amenable to conventional DNA manipulations. The method involves ligation-independent …

The last four years have brought exciting progress in membrane protein research. Finally
those many efforts that have been put into expression of eukaryotic membrane proteins are …

Multidrug and toxic compound extrusion (MATE) transporters mediate excretion of
xenobiotics and toxic metabolites, thereby conferring multidrug resistance in bacterial …

Fluorescence spectroscopy has been a cornerstone of research in membrane dynamics and
organization. Technological advances in fluorescence spectroscopy went hand in hand with …