Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …

The analysis of peptides and proteins as well as the grander scope of proteomics (large
scale study of proteins) has been advanced by the development of a versatile array of ion …

(MS) and MS-driven proteomics have continued to develop as the premier tools for
characterizing proteome composition, structure, and function. 2 The new era of proteomics …

Highlights•Histidine phosphorylation (pHis) is reversible by specific kinases and
phosphatases.•pHis is heat and acid labile, but stable under alkaline conditions.•Stable pHis …

Here we present IPSA, an innovative web-based spectrum annotator that visualizes and
characterizes peptide tandem mass spectra. A tool for the scientific community, IPSA can …

Pioneering work by, among others, the groups of Hunt and Mann at the start of this century
opened up the era of mass spectrometry (MS)-based phosphoproteomics. 1− 3 Through …

Label-free protein characterization at surfaces is commonly achieved using digestion and/or
matrix application prior to mass spectrometry. We report the assignment of undigested …

The proteome, or collection of proteoforms expressed in a biological system, is dynamic and
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …

At mammalian neuronal synapses, synaptic vesicle (SV) glycoproteins are essential for
robust neurotransmission. Asparagine (N)-linked glycosylation is required for delivery of the …