Vacuolar processing enzymes in the plant life cycle
K Yamada, AK Basak, S Goto‐Yamada… - New …, 2020 - Wiley Online Library
Vacuolar processing enzyme (VPE) is a cysteine‐type endopeptidase that has a substrate‐
specificity for asparagine or aspartic acid residues and cleaves peptide bonds at their …
specificity for asparagine or aspartic acid residues and cleaves peptide bonds at their …
Plant vacuolar processing enzymes
Plant proteomes contain hundreds of proteases divided into different families based on
evolutionary and functional relationship. In particular, plant cysteine proteases of the C1 …
evolutionary and functional relationship. In particular, plant cysteine proteases of the C1 …
Molecular basis for the production of cyclic peptides by plant asparaginyl endopeptidases
Asparaginyl endopeptidases (AEPs) are proteases that have crucial roles in plant defense
and seed storage protein maturation. Select plant AEPs, however, do not function as …
and seed storage protein maturation. Select plant AEPs, however, do not function as …
Structural determinants for peptide-bond formation by asparaginyl ligases
Asparaginyl endopeptidases (AEPs) are cysteine proteases which break Asx (Asn/Asp)–Xaa
bonds in acidic conditions. Despite sharing a conserved overall structure with AEPs, certain …
bonds in acidic conditions. Despite sharing a conserved overall structure with AEPs, certain …
A bifunctional asparaginyl endopeptidase efficiently catalyzes both cleavage and cyclization of cyclic trypsin inhibitors
J Du, K Yap, LY Chan, FBH Rehm, FY Looi… - Nature …, 2020 - nature.com
Asparaginyl endopeptidases (AEPs) catalyze the key backbone cyclization step during the
biosynthesis of plant-derived cyclic peptides. Here, we report the identification of two AEPs …
biosynthesis of plant-derived cyclic peptides. Here, we report the identification of two AEPs …
Sensitive determination of proteolytic proteoforms in limited microscale proteome samples
Protein N termini unambiguously identify truncated, alternatively translated or modified
proteoforms with distinct functions and reveal perturbations in disease. Selective enrichment …
proteoforms with distinct functions and reveal perturbations in disease. Selective enrichment …
The macrocyclizing protease butelase 1 remains autocatalytic and reveals the structural basis for ligase activity
AM James, J Haywood, J Leroux, K Ignasiak… - The Plant …, 2019 - Wiley Online Library
Plant asparaginyl endopeptidases (AEP s) are expressed as inactive zymogens that perform
maturation of seed storage protein upon cleavage‐dependent autoactivation in the low‐pH …
maturation of seed storage protein upon cleavage‐dependent autoactivation in the low‐pH …
pH-controlled protein orthogonal ligation using asparaginyl peptide ligases
Peptide asparaginyl ligases (PALs) catalyze transpeptidation at the Asn residue of a short
Asn-Xaa1-Xaa2 tripeptide motif. Due to their high catalytic activity toward the P1-Asn …
Asn-Xaa1-Xaa2 tripeptide motif. Due to their high catalytic activity toward the P1-Asn …
The asparaginyl endopeptidase legumain: an emerging therapeutic target and potential biomarker for Alzheimer's disease
M Song - International Journal of Molecular Sciences, 2022 - mdpi.com
Alzheimer's disease (AD) is incurable dementia closely associated with aging. Most cases of
AD are sporadic, and very few are inherited; the pathogenesis of sporadic AD is complex …
AD are sporadic, and very few are inherited; the pathogenesis of sporadic AD is complex …
A suite of kinetically superior AEP ligases can cyclise an intrinsically disordered protein
KS Harris, RF Guarino, RS Dissanayake, P Quimbar… - Scientific reports, 2019 - nature.com
Asparaginyl endopeptidases (AEPs) are a class of enzymes commonly associated with
proteolysis in the maturation of seed storage proteins. However, a subset of AEPs work …
proteolysis in the maturation of seed storage proteins. However, a subset of AEPs work …