High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives
The ease of genetic manipulation, low cost, rapid growth and number of previous studies
have made Escherichia coli one of the most widely used microorganism species for …
have made Escherichia coli one of the most widely used microorganism species for …
High-throughput process development from gene cloning to protein production
M Sun, AX Gao, X Liu, Y Yang, R Ledesma-Amaro… - Microbial Cell …, 2023 - Springer
In the post-genomic era, the demand for faster and more efficient protein production has
increased, both in public laboratories and industry. In addition, with the expansion of protein …
increased, both in public laboratories and industry. In addition, with the expansion of protein …
A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis
K Motohashi - BMC biotechnology, 2015 - Springer
Background Seamless ligation cloning extract (SLiCE) is a simple and efficient method for
DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses …
DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses …
A green pathway for lignin valorization: Enzymatic lignin depolymerization in biocompatible ionic liquids and deep eutectic solvents
Lignin depolymerization, which enables the breakdown of a complex and heterogeneous
aromatic polymer into relatively uniform derivatives, serves as a critical process in …
aromatic polymer into relatively uniform derivatives, serves as a critical process in …
Carbohydrate binding modules enhance cellulose enzymatic hydrolysis by increasing access of cellulases to the substrate
Plant biomass is a low-cost and abundant source of carbohydrates for production of
fuels,“green” chemicals and materials. Currently, biochemical conversion of the biomass into …
fuels,“green” chemicals and materials. Currently, biochemical conversion of the biomass into …
[PDF][PDF] Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide …
AGV Sepulchro, VOA Pellegrini, LD Dias… - Biofuel Research …, 2021 - repositorio.usp.br
3. Results and Discussion.......................................................................................................................
......................................................................................... 3.1. Mt LPMO9A sequence …
......................................................................................... 3.1. Mt LPMO9A sequence …
[HTML][HTML] A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit
Y Okegawa, K Motohashi - Biochemistry and Biophysics Reports, 2015 - Elsevier
The seamless ligation cloning extract (SLiCE) method is a novel seamless DNA cloning tool
that utilizes homologous recombination activities in Escherichia coli cell lysates to assemble …
that utilizes homologous recombination activities in Escherichia coli cell lysates to assemble …
A family of AA9 lytic polysaccharide monooxygenases in Aspergillus nidulans is differentially regulated by multiple substrates and at least one is active on cellulose …
G Jagadeeswaran, L Gainey, R Prade… - Applied Microbiology and …, 2016 - Springer
Fungal genomes contain multiple genes encoding AA9 lytic polysaccharide
monooxygenases (LPMOs), a recently discovered class of enzymes known to be active on …
monooxygenases (LPMOs), a recently discovered class of enzymes known to be active on …
Paludibacter propionicigenes GH10 xylanase as a tool for enzymatic xylooligosaccharides production from heteroxylans
Bioconversion of lignocellulosic biomass into value-added products relies on
polysaccharides depolymerization by carbohydrate active enzymes. This work reports …
polysaccharides depolymerization by carbohydrate active enzymes. This work reports …
Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase
Enzymatic transformation of xylans into renewable fuels and value-added products is
mediated by xylanases. Here we describe the biochemical and X-ray structural …
mediated by xylanases. Here we describe the biochemical and X-ray structural …