Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts

P Patra, M Das, P Kundu, A Ghosh - Biotechnology Advances, 2021 - Elsevier
Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon
sources is currently an advancing area in industrial research. The model yeast …

[HTML][HTML] Metabolic engineering of Pichia pastoris

DA Peña, B Gasser, J Zanghellini, MG Steiger… - Metabolic …, 2018 - Elsevier
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae

MI Espinosa, RA Gonzalez-Garcia, K Valgepea… - Nature …, 2020 - nature.com
Utilising one-carbon substrates such as carbon dioxide, methane, and methanol is vital to
address the current climate crisis. Methylotrophic metabolism enables growth and energy …

[HTML][HTML] Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

J Gao, L Jiang, J Lian - Synthetic and systems biotechnology, 2021 - Elsevier
The methylotrophic yeast Pichia pastoris (aka Komagataella phaffii) is one of the most
commonly used hosts for industrial production of recombinant proteins. As a non …

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications

P Cai, J Gao, Y Zhou - Microbial cell factories, 2019 - Springer
Non-conventional yeasts are playing important roles as cell factories for bioproduction of
biofuels, food additives and proteins with outstanding natural characteristics. However, the …

Bio-synthesis of food additives and colorants-a growing trend in future food

L Sun, F Xin, HS Alper - Biotechnology Advances, 2021 - Elsevier
Food additives and colorants are extensively used in the food industry to improve food
quality and safety during processing, storage and packing. Sourcing of these molecules is …

Advances in developing metabolically engineered microbial platforms to produce fourth-generation biofuels and high-value biochemicals

MA Mehmood, A Shahid, S Malik, N Wang… - Bioresource …, 2021 - Elsevier
Producing bio-based chemicals is imperative to establish an eco-friendly circular
bioeconomy. However, the compromised titer of these biochemicals hampers their …

Bioengineered microbial platforms for biomass-derived biofuel production–A review

H Lu, V Yadav, M Zhong, M Bilal, MJ Taherzadeh… - Chemosphere, 2022 - Elsevier
Global warming issues, rapid fossil fuel diminution, and increasing worldwide energy
demands have diverted accelerated attention in finding alternate sources of biofuels and …

Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris

X Jin, W Zhang, Y Wang, J Sheng, R Xu, J Li, G Du… - Green …, 2021 - pubs.rsc.org
Chondroitin sulfate (CS) is widely applied in the medical, clinical, and nutraceutical fields.
However, all commercialized CS is extracted from animal tissues, which is associated with …

Microbial engineering for the production of isobutanol: current status and future directions

NM Lakshmi, P Binod, R Sindhu, MK Awasthi… - …, 2021 - Taylor & Francis
Fermentation-derived alcohols have gained much attention as an alternate fuel due to its
minimal effects on atmosphere. Besides its application as biofuel it is also used as raw …