Radiation damage to biological macromolecules∗

EF Garman, M Weik - Current Opinion in Structural Biology, 2023 - Elsevier
In this review, we describe recent research developments into radiation damage effects in
macromolecular X-ray crystallography observed at synchrotrons and X-ray free electron …

The red edge: bilin-binding photoreceptors as optogenetic tools and fluorescence reporters

K Tang, HM Beyer, MD Zurbriggen… - Chemical …, 2021 - ACS Publications
This review adds the bilin-binding phytochromes to the Chemical Reviews thematic issue
“Optogenetics and Photopharmacology”. The work is structured into two parts. We first …

Plant phytochrome B is an asymmetric dimer with unique signalling potential

H Li, ES Burgie, ZTK Gannam, H Li, RD Vierstra - Nature, 2022 - nature.com
Many aspects of plant photoperception are mediated by the phytochrome (Phy) family of
bilin-containing photoreceptors that reversibly interconvert between inactive Pr and active …

An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography

A Butryn, PS Simon, P Aller, P Hinchliffe… - Nature …, 2021 - nature.com
Serial femtosecond crystallography has opened up many new opportunities in structural
biology. In recent years, several approaches employing light-inducible systems have …

A guide to time‐resolved structural analysis of light‐activated proteins

H Poddar, DJ Heyes, G Schirò, M Weik… - The FEBS …, 2022 - Wiley Online Library
Dynamical changes in protein structures are essential for protein function and occur over
femtoseconds to seconds timescales. X‐ray free electron lasers have facilitated …

Tips and turns of bacteriophytochrome photoactivation

H Takala, P Edlund, JA Ihalainen… - Photochemical & …, 2020 - pubs.rsc.org
Phytochromes are ubiquitous photosensor proteins, which control the growth, reproduction
and movement in plants, fungi and bacteria. Phytochromes switch between two …

Bistable photoswitch allows in vivo control of hematopoiesis

L Albert, J Nagpal, W Steinchen, L Zhang… - ACS central …, 2021 - ACS Publications
Optical control has enabled functional modulation in cell culture with unparalleled
spatiotemporal resolution. However, current tools for in vivo manipulation are scarce. Here …

The structure of Arabidopsis phytochrome A reveals topological and functional diversification among the plant photoreceptor isoforms

ES Burgie, H Li, ZTK Gannam, KE McLoughlin… - Nature plants, 2023 - nature.com
Plants employ a divergent cohort of phytochrome (Phy) photoreceptors to govern many
aspects of morphogenesis through reversible photointerconversion between inactive Pr and …

Signaling by a bacterial phytochrome histidine kinase involves a conformational cascade reorganizing the dimeric photoreceptor

ES Burgie, K Basore, MJ Rau, B Summers… - Nature …, 2024 - nature.com
Phytochromes (Phys) are a divergent cohort of bili-proteins that detect light through
reversible interconversion between dark-adapted Pr and photoactivated Pfr states. While our …

Green/red light-sensing mechanism in the chromatic acclimation photosensor

T Nagae, Y Fujita, T Tsuchida, T Kamo, R Seto… - Science …, 2024 - science.org
Certain cyanobacteria alter their photosynthetic light absorption between green and red, a
phenomenon called complementary chromatic acclimation. The acclimation is regulated by …