Synthetic protein condensates for cellular and metabolic engineering

ZG Qian, SC Huang, XX Xia - Nature chemical biology, 2022 - nature.com
Protein condensates are distinct structures assembled in living cells that concentrate
molecules via phase separation in a confined subcellular compartment. In the past decade …

P450-driven plastic-degrading synthetic bacteria

SJ Yeom, TK Le, CH Yun - Trends in Biotechnology, 2022 - cell.com
Plastic contamination currently threatens a wide variety of ecosystems and presents
damaging repercussions and negative consequences for many wildlife species. Sustainable …

Engineered Artificial Membraneless Organelles in Saccharomyces cerevisiae To Enhance Chemical Production

P Zhou, H Liu, X Meng, H Zuo, M Qi… - Angewandte Chemie …, 2023 - Wiley Online Library
Microbial cell factories provide a green and sustainable opportunity to produce value‐added
products from renewable feedstock. However, the leakage of toxic or volatile intermediates …

Engineering status of protein for improving microbial cell factories

P Zhou, C Gao, W Song, W Wei, J Wu, L Liu… - Biotechnology …, 2024 - Elsevier
With the development of metabolic engineering and synthetic biology, microbial cell
factories (MCFs) have provided an efficient and sustainable method to synthesize a series of …

Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440

D Salvachúa, AZ Werner, I Pardo… - Proceedings of the …, 2020 - National Acad Sciences
Lignin is an abundant and recalcitrant component of plant cell walls. While lignin
degradation in nature is typically attributed to fungi, growing evidence suggests that bacteria …

A “push-pull-restrain” strategy to improve citronellol production in Saccharomyces cerevisiae

G Jiang, M Yao, Y Wang, W Xiao, Y Yuan - Metabolic Engineering, 2021 - Elsevier
Microbial production of monoterpenes has attracted increasing attention in recent years. Up
to date, there are only few reports on the biosynthesis of the monoterpene alcohol citronellol …

Reductive cytochrome P450 reactions and their potential role in bioremediation

JBYH Behrendorff - Frontiers in Microbiology, 2021 - frontiersin.org
Cytochrome P450 enzymes, or P450s, are haem monooxygenases renowned for their ability
to insert one atom from molecular oxygen into an exceptionally broad range of substrates …

Emerging strategies for modifying lignin chemistry to enhance biological lignin valorization

ZM Zhao, ZH Liu, Y Pu, X Meng, J Xu, JS Yuan… - …, 2020 - Wiley Online Library
Biological lignin valorization represents a promising approach contributing to sustainable
and economic biorefineries. The low level of valuable lignin‐derived products remains a …

Synthetic protein scaffolds for the colocalisation of co-acting enzymes

J Vanderstraeten, Y Briers - Biotechnology Advances, 2020 - Elsevier
Nature relies on complexes of colocated enzymes to efficiently perform multiple catalytic
steps. Such enzyme colocalisation promotes substrate channelling, enhances the activity of …

De novo design of a reversible phosphorylation-dependent switch for membrane targeting

L Harrington, JM Fletcher, T Heermann… - Nature …, 2021 - nature.com
Modules that switch protein-protein interactions on and off are essential to develop synthetic
biology; for example, to construct orthogonal signaling pathways, to control artificial protein …