Materials functionalization with multicomponent reactions: state of the art

R Afshari, A Shaabani - ACS Combinatorial Science, 2018 - ACS Publications
The emergence of neoteric synthetic routes for materials functionalization is an interesting
phenomenon in materials chemistry. In particular, the union of materials chemistry with …

Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry

MM Gomari, N Saraygord-Afshari, M Farsimadan… - Biotechnology …, 2020 - Elsevier
Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …

Affinity tags in protein purification and peptide enrichment: an overview

AS Pina, ÍL Batalha, AMGC Dias… - … Design, Development, and …, 2021 - Springer
The reversible interaction between an affinity ligand and a complementary receptor has
been widely explored in purification systems for several biomolecules. The development of …

Rational design of affinity ligands for bioseparation

MJB Matos, AS Pina, ACA Roque - Journal of Chromatography A, 2020 - Elsevier
Affinity adsorbents have been the cornerstone in protein purification. The selective nature of
the molecular recognition interactions established between an affinity ligands and its target …

Petasis-Ugi ligands: New affinity tools for the enrichment of phosphorylated peptides

ÍL Batalha, ACA Roque - Journal of Chromatography B, 2016 - Elsevier
Affinity chromatography is a widespread technique for the enrichment and isolation of
biologics, which relies on the selective and reversible interaction between affinity ligands …

Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins

AS Pina, S Carvalho, AMGC Dias, M Guilherme… - … of Chromatography A, 2016 - Elsevier
A common strategy for the production and purification of recombinant proteins is to fuse a
tag to the protein terminal residues and employ a “tag-specific” ligand for fusion protein …

New multimodal stationary phases prepared by Ugi multicomponent approach

D Sýkora, K Záruba, M Butnariu, A Tatar… - Journal of …, 2020 - Wiley Online Library
Eight different stationary phases based on two aminopropyl silicas of different brands
suitable for multimodal chromatography applications have been prepared by a four …

Mimicking nature: Phosphopeptide enrichment using combinatorial libraries of affinity ligands

IL Batalha, H Zhou, K Lilley, CR Lowe… - … of Chromatography A, 2016 - Elsevier
Phosphorylation is a reversible post-translational modification of proteins that controls a
plethora of cellular processes and triggers specific physiological responses, for which there …

Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent …

Y Kaveh-Baghbaderani, SA Blank-Shim, T Koch… - Protein Expression and …, 2018 - Elsevier
The need for fast and convenient protein purification is enormous, not only on the industrial
scale for the production of therapeutics but also in life science research. Most protein …

Biomimetic Affinity Ligands for Protein Purification

IT Sousa, MÂ Taipa - … Processing: Design, Development, and Application of …, 2021 - Springer
The development of sophisticated molecular modeling software and new bioinformatic tools,
as well as the emergence of data banks containing detailed information about a huge …