With the development of molecular marker technology in the 1980s, the fate of plant
breeding has changed. Different types of molecular markers have been developed and …

The polymerase chain reaction (PCR) has dramatically altered how molecular studies are
conducted as well as what questions can be asked. In addition to simplifying molecular tasks …

An efficient genome-scale editing tool is required for construction of industrially useful
microbes. We describe a targeted, continual multigene editing strategy that was applied to …

Cell-based biosensors have great potential to detect various toxic and pathogenic
contaminants in aqueous environments. However, frequently they cannot meet practical …

Background The ability to selectively alter genomic DNA sequences in vivo is a powerful tool
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …

Various in vivo mutagenesis methods have been developed to facilitate fast and efficient
continuous evolution of proteins in cells. However, they either modify the DNA region that …

Bacteria and Archaea have developed several defence strategies against foreign nucleic
acids such as viral genomes and plasmids. Among them, clustered regularly interspaced …

We set out to exhaustively characterize the impact of the cis-chromatin environment on
prime editing, a precise genome engineering tool. Using a highly sensitive method for …

Genome‐wide screens have discovered a large set of essential genes in the opportunistic
human pathogen Streptococcus pneumoniae. However, the functions of many essential …

Genetic and metabolic studies have been done on a large kindred in which several males
are affected by a syndrome of borderline mental retardation and abnormal behavior. The …