Developing recombinant protein pharmaceuticals has proved to be very challenging
because of both the complexity of protein production and purification, and the limited …

Thermophilic proteins show substantially higher intrinsic thermal stability than their
mesophilic counterparts. Amino acid composition is believed to alter the intrinsic stability of …

We have examined the architecture of a protein complex in the absence of bulk water. By
determining collision cross sections of assemblies of the trp RNA binding protein, TRAP, we …

The homodimeric HIV-1 protease is the target of some of the most effective antiviral AIDS
therapy, as it facilitates viral maturation by cleaving ten asymmetric and nonhomologous …

Few organisms are able to withstand desiccation stress; however, desiccation tolerance is
widespread among plant seeds. Survival without water relies on an array of mechanisms …

Protein–water interactions are key to biological function. They have an underlying dynamic
component that pervades the functional roles associated both with particular systems and …

Biologically relevant macromolecules, such as proteins, do not operate as static, isolated
entities. On the contrary, they are involved in numerous interactions with other species, such …

Preparation of proteins in their crystalline state has been found to be important in producing
stable therapeutic protein formulations, cross-linked enzyme crystals for application in …

Hydrophilins are a wide group of proteins whose defining characteristics are high
hydrophilicity index (> 1.0) and high glycine content (> 6%). The transcripts of most …

During electrospray ionization (ESI), proteins are transferred from solution into vacuum, a
process that influences the conformation of the protein. Exactly how much the conformation …