Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: a methods overview

CL Mummery, J Zhang, ES Ng, DA Elliott… - Circulation …, 2012 - Am Heart Assoc
Since human embryonic stem cells were first differentiated to beating cardiomyocytes a
decade ago, interest in their potential applications has increased exponentially. This has …

Bioprinting for stem cell research

S Tasoglu, U Demirci - Trends in biotechnology, 2013 - cell.com
Recently, there has been growing interest in applying bioprinting techniques to stem cell
research. Several bioprinting methods have been developed utilizing acoustics …

Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

X Lian, J Zhang, SM Azarin, K Zhu, LB Hazeltine… - Nature protocols, 2013 - nature.com
The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to
functional cardiomyocytes in a completely defined, growth factor–and serum-free system by …

A simple bioreactor-based method to generate kidney organoids from pluripotent stem cells

A Przepiorski, V Sander, T Tran, JA Hollywood… - Stem Cell Reports, 2018 - cell.com
Kidney organoids made from pluripotent stem cells have the potential to revolutionize how
kidney development, disease, and injury are studied. Current protocols are technically …

Cellular and engineered organoids for cardiovascular models

D Thomas, S Choi, C Alamana, KK Parker… - Circulation …, 2022 - Am Heart Assoc
An ensemble of in vitro cardiac tissue models has been developed over the past several
decades to aid our understanding of complex cardiovascular disorders using a reductionist …

Development of a valve-based cell printer for the formation of human embryonic stem cell spheroid aggregates

A Faulkner-Jones, S Greenhough, JA King… - …, 2013 - iopscience.iop.org
In recent years, the use of a simple inkjet technology for cell printing has triggered
tremendous interest and established the field of biofabrication. A key challenge has been …

Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells

P Hoang, J Wang, BR Conklin, KE Healy, Z Ma - Nature protocols, 2018 - nature.com
The creation of human induced pluripotent stem cells (hiPSCs) has provided an
unprecedented opportunity to study tissue morphogenesis and organ development through' …

Engineered microsystems for spheroid and organoid studies

SM Kang, D Kim, JH Lee, S Takayama… - Advanced healthcare …, 2021 - Wiley Online Library
Abstract 3D in vitro model systems such as spheroids and organoids provide an opportunity
to extend the physiological understanding using recapitulated tissues that mimic …

Human pluripotent stem cells: an emerging model in developmental biology

Z Zhu, D Huangfu - Development, 2013 - journals.biologists.com
Developmental biology has long benefited from studies of classic model organisms.
Recently, human pluripotent stem cells (hPSCs), including human embryonic stem cells and …

In vitro cardiac tissue models: current status and future prospects

A Mathur, Z Ma, P Loskill, S Jeeawoody… - Advanced drug delivery …, 2016 - Elsevier
Cardiovascular disease is the leading cause of death worldwide. Achieving the next phase
of potential treatment strategies and better prognostic tools will require a concerted effort …