▪ Abstract DNA replication fidelity is a key determinant of genome stability and is central to
the evolution of species and to the origins of human diseases. Here we review our current …

Binuclear metallohydrolases are a structurally diverse group of enzymes that use binuclear
metal ion centers to catalyze the hydrolysis of amides and esters of carboxylic and …

Remdesivir is a nucleoside analog approved by the US FDA for treatment of COVID-19.
Here, we present a 3.9-Å-resolution cryo-EM reconstruction of a remdesivir-stalled RNA …

DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle,
while achieving error frequencies in the range of 10− 5to 10− 6. Here we present a 2.2 Å …

The fidelity of DNA polymerases is largely attributable to a two-step nucleotide binding
mechanism. In the first step, binding contacts are initially made between the template and …

Live, attenuated RNA virus vaccines are efficacious but subject to reversion to virulence.
Among RNA viruses, replication fidelity is recognized as a key determinant of virulence and …

The mechanism of inhibition of HIV-1 reverse transcriptase by three nonnucleoside
inhibitors is described. Nevirapine, O-TIBO, and CI-TIBO each bind to a hydrophobic pocket …

Abstract T7 DNA polymerase, catalyses DNA replication in vitro at rates of more than 100
bases per second and has a 3′→ 5′ exonuclease (nucleotide removing) activity at a …

We describe the 2.6 Å resolution crystal structure of RB69 DNA polymerase with primer-
template DNA and dTTP, capturing the step just before primer extension. This ternary …

Integration of DNA isolation, amplification, and sequencing can be achieved by the use of
polymerase colonies (polonies) and cycles of fluorescent dNTP incorporation. In this paper …