Regulation of Pichia pastoris promoters and its consequences for protein production

T Vogl, A Glieder - New biotechnology, 2013 - Elsevier
The methylotrophic yeast Pichia pastoris is a widely used host for heterologous protein
production. Along with favorable properties such as growth to high cell density and high …

Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins

H Raschmanová, A Weninger, Z Knejzlík… - Applied Microbiology …, 2021 - Springer
Folding and processing of proteins in the endoplasmic reticulum (ER) are major
impediments in the production and secretion of proteins from Pichia pastoris (Komagataella …

Molecular Healing of Polymeric Materials, Coatings, Plastics, Elastomers, Composites, Laminates, Adhesives, and Sealants by Active Enzymes

CS McDaniel, ME Wales, J Rawlins, P Cipi… - US Patent App. 12 …, 2010 - Google Patents
2010-04-30 Assigned to REACTIVE SURFACES, LTD. reassignment REACTIVE
SURFACES, LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR …

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

B Gasser, M Saloheimo, U Rinas, M Dragosits… - Microbial cell …, 2008 - Springer
Different species of microorganisms including yeasts, filamentous fungi and bacteria have
been used in the past 25 years for the controlled production of foreign proteins of scientific …

Systems biotechnology for protein production in Pichia pastoris

RJ Zahrl, DA Peña, D Mattanovich… - FEMS yeast …, 2017 - academic.oup.com
The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most
important production systems for heterologous proteins. After the first genome sequences …

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris

V Puxbaum, D Mattanovich, B Gasser - Applied microbiology and …, 2015 - Springer
The development of Pichia pastoris as a production platform for recombinant proteins has
been a remarkable success story over the last three decades. Stable cheap production …

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

M Guerfal, S Ryckaert, PP Jacobs, P Ameloot… - Microbial cell …, 2010 - Springer
Background The unfolded protein response (UPR) in eukaryotes upregulates factors that
restore ER homeostasis upon protein folding stress and in yeast is activated by a non …

Systems-level organization of yeast methylotrophic lifestyle

H Rußmayer, M Buchetics, C Gruber, M Valli… - BMC biology, 2015 - Springer
Background Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize
the single carbon compound methanol as a carbon and energy source. Among them, Pichia …

Pathway engineering facilitates efficient protein expression in Pichia pastoris

C Liu, JS Gong, C Su, H Li, H Li, ZM Rao, ZH Xu… - Applied Microbiology …, 2022 - Springer
Pichia pastoris has been recognized as an important platform for the production of various
heterologous proteins in recent years. The strong promoter AOX1, induced by methanol …

Efficient generation of multi‐copy strains for optimizing secretory expression of porcine insulin precursor in yeast Pichia pastoris

T Zhu, M Guo, Z Tang, M Zhang… - Journal of applied …, 2009 - academic.oup.com
Aims: This study attempted to fully explore the expression potentials of Pichia pastoris for
producing porcine insulin precursor (PIP) through PIP copy number optimization. Methods …