Synthetic genomics is the construction of viruses, bacteria, and eukaryotic cells with
synthetic genomes. It involves two basic processes: synthesis of complete genomes or …

Gene expression is regulated by transcription factors that work together to read cis-
regulatory DNA sequences. The 'cis-regulatory code'—how cells interpret DNA sequences …

Genetically engineered mouse models (GEMMs) help us to understand human pathologies
and develop new therapies, yet faithfully recapitulating human diseases in mice is …

Here, we report the design, construction, and characterization of a tRNA neochromosome, a
designer chromosome that functions as an additional, de novo counterpart to the native …

We describe construction of the synthetic yeast chromosome XI (synXI) and reveal the
effects of redesign at non-coding DNA elements. The 660-kb synthetic yeast genome project …

Chromosome-level design-build-test-learn cycles (chrDBTLs) allow systematic
combinatorial reconfiguration of chromosomes with ease. Here, we established chrDBTL …

Pioneering advances in genome engineering, and specifically in genome writing, have
revolutionized the field of synthetic biology, propelling us toward the creation of synthetic …

Aneuploidy compromises genomic stability, often leading to embryo inviability, and is
frequently associated with tumorigenesis and aging. Different aneuploid chromosome …

We describe the complete synthesis, assembly, debugging, and characterization of a
synthetic 404,963 bp chromosome, synIX (synthetic chromosome IX). Combined …

The de novo construction of a living organism is a compelling vision. Despite the astonishing
technologies developed to modify living cells, building a functioning cell “from scratch” has …