[HTML][HTML] Probing allosteric communication with combined molecular dynamics simulations and network analysis
Understanding the allosteric mechanisms within biomolecules involved in diseases is of
paramount importance for drug discovery. Indeed, characterizing communication pathways …
paramount importance for drug discovery. Indeed, characterizing communication pathways …
Unveiling the RNA-mediated allosteric activation discloses functional hotspots in CRISPR-Cas13a
S Sinha, AM Molina Vargas, PR Arantes… - Nucleic Acids …, 2024 - academic.oup.com
Cas13a is a recent addition to the CRISPR-Cas toolkit that exclusively targets RNA, which
makes it a promising tool for RNA detection. It utilizes a CRISPR RNA (crRNA) to target RNA …
makes it a promising tool for RNA detection. It utilizes a CRISPR RNA (crRNA) to target RNA …
Programmable RNA targeting with CRISPR-Cas13
P Shi, X Wu - RNA biology, 2024 - Taylor & Francis
ABSTRACT The RNA-targeting CRISPR-Cas13 system has enabled precise engineering of
endogenous RNAs, significantly advancing our understanding of RNA regulation and the …
endogenous RNAs, significantly advancing our understanding of RNA regulation and the …
CRISPR/Cas12a-Powered Amplification-Free RNA Diagnostics by Integrating T7 Exonuclease-Assisted Target Recycling and Split G-Quadruplex Catalytic Signal …
D Zhang, B Tian, Y Ling, L Ye, M Xiao, K Yuan… - Analytical …, 2024 - ACS Publications
Rapid and sensitive RNA detection is of great value in diverse areas, ranging from
biomedical research to clinical diagnostics. Existing methods for RNA detection often rely on …
biomedical research to clinical diagnostics. Existing methods for RNA detection often rely on …
Dynamic Addressing Molecular Robot (DAMR): An Effective and Efficient Trial‐and‐Error Approach for the Analysis of Single Nucleotide Polymorphisms
T Chen, G Chen, S Cao, X Tang, W Li, C Liu… - Advanced …, 2024 - Wiley Online Library
Accurate and efficient molecular recognition plays a crucial role in the fields of molecular
detection and diagnostics. Conventional trial‐and‐error‐based molecular recognition …
detection and diagnostics. Conventional trial‐and‐error‐based molecular recognition …
Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics
Q Wu, Z Yi, H Li, G Han, J Du, J Xiong, K Hu, H Gao - 2024 - researchsquare.com
Cas12 and Cas13 are extensively utilized in molecular diagnostics for their trans-cleavage
activities, yet their activation characteristics remain partially understood. Here, we conduct …
activities, yet their activation characteristics remain partially understood. Here, we conduct …