In vitro selection of aptamers and their applications

MC DeRosa, A Lin, P Mallikaratchy… - Nature Reviews …, 2023 - nature.com
The introduction of the in vitro evolution method known as SELEX (systematic evolution of
ligands by exponential enrichment) more than 30 years ago led to the conception of …

Advances and challenges in small‐molecule DNA aptamer isolation, characterization, and sensor development

H Yu, O Alkhamis, J Canoura, Y Liu… - Angewandte Chemie …, 2021 - Wiley Online Library
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind
to specific molecules with high affinity, and offer a number of advantages relative to …

Computational tools for aptamer identification and optimization

D Sun, M Sun, J Zhang, X Lin, Y Zhang, F Lin… - TrAC Trends in …, 2022 - Elsevier
Aptamers are single-stranded DNA or RNA oligonucleotides that can selectively bind to a
specific target. They are generally obtained by SELEX, but the procedure is challenging and …

Modified nucleic acid aptamers: development, characterization, and biological applications

D Ji, H Feng, SW Liew, CK Kwok - Trends in Biotechnology, 2023 - cell.com
Aptamers are single-stranded oligonucleotides that bind to their targets via specific structural
interactions. To improve the properties and performance of aptamers, modified nucleotides …

Design and prediction of aptamers assisted by in silico methods

SJ Lee, J Cho, BH Lee, D Hwang, JW Park - Biomedicines, 2023 - mdpi.com
An aptamer is a single-stranded DNA or RNA that binds to a specific target with high binding
affinity. Aptamers are developed through the process of systematic evolution of ligands by …

[Retracted] MicroRNA‐133b Inhibition Restores EGFR Expression and Accelerates Diabetes‐Impaired Wound Healing

H Zhong, J Qian, Z Xiao, Y Chen, X He… - Oxidative Medicine …, 2021 - Wiley Online Library
Diabetic foot ulcers (DFUs) are caused by impairments in peripheral blood vessel
angiogenesis and represent a great clinical challenge. Although various innovative …

On the move-sensitive fluorescent aptassay on board catalytic micromotors for the determination of interleukin-6 in ultra-low serum volumes for neonatal sepsis …

J Gordón, L Arruza, MD Ibáñez, M Moreno-Guzmán… - ACS …, 2022 - ACS Publications
A graphene oxide/nickel/platinum nanoparticle micromotor (MM)-based fluorescent
aptassay is proposed to determine interleukin-6 (IL-6) in serum samples from low-birth …

Nucleic acid aptamers as aptasensors for plant biology

S Tungsirisurp, R O'Reilly, R Napier - Trends in Plant Science, 2023 - cell.com
Our knowledge of cell-and tissue-specific quantification of phytohormones is heavily reliant
on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed …

Implementation of high-throughput sequencing (HTS) in aptamer selection technology

N Komarova, D Barkova, A Kuznetsov - International journal of molecular …, 2020 - mdpi.com
Aptamers are nucleic acid ligands that bind specifically to a target of interest. Aptamers have
gained in popularity due to their high potential for different applications in analysis …

Generating experimentally unrelated target molecule-binding highly functionalized nucleic-acid polymers using machine learning

JC Chen, JP Chen, MW Shen, M Wornow… - Nature …, 2022 - nature.com
In vitro selection queries large combinatorial libraries for sequence-defined polymers with
target binding and reaction catalysis activity. While the total sequence space of these …