CRISPR-Cas9 system: A new-fangled dawn in gene editing
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Anti-trypanosomatid drug discovery: an ongoing challenge and a continuing need
The WHO recognizes human African trypanosomiasis, Chagas disease and the
leishmaniases as neglected tropical diseases. These diseases are caused by parasitic …
leishmaniases as neglected tropical diseases. These diseases are caused by parasitic …
[HTML][HTML] A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes
Apicomplexan parasites are leading causes of human and livestock diseases such as
malaria and toxoplasmosis, yet most of their genes remain uncharacterized. Here, we …
malaria and toxoplasmosis, yet most of their genes remain uncharacterized. Here, we …
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems
Programmable nucleases enable engineering of the genome by utilizing endogenous DNA
double-strand break (DSB) repair pathways. Although homologous recombination (HR) …
double-strand break (DSB) repair pathways. Although homologous recombination (HR) …
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids
T Beneke, R Madden, L Makin… - Royal Society …, 2017 - royalsocietypublishing.org
Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
Although CRISPR/Cas9 genome editing has provided numerous opportunities to interrogate
the functional significance of any given genomic site, there is a paucity of data on the extent …
the functional significance of any given genomic site, there is a paucity of data on the extent …
Spontaneous dormancy protects Trypanosoma cruzi during extended drug exposure
FJ Sánchez-Valdéz, A Padilla, W Wang, D Orr… - Elife, 2018 - elifesciences.org
The ability of the Chagas disease agent Trypanosoma cruzi to resist extended in vivo
exposure to highly effective trypanocidal compounds prompted us to explore the potential for …
exposure to highly effective trypanocidal compounds prompted us to explore the potential for …
Efficient disruption and replacement of an effector gene in the oomycete Phytophthora sojae using CRISPR/Cas9
P hytophthora sojae is an oomycete pathogen of soybean. As a result of its economic
importance, P. sojae has become a model for the study of oomycete genetics, physiology …
importance, P. sojae has become a model for the study of oomycete genetics, physiology …
EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens
D Peng, R Tarleton - Microbial genomics, 2015 - microbiologyresearch.org
Recent development of CRISPR-Cas9 genome editing has enabled highly efficient and
versatile manipulation of a variety of organisms and adaptation of the CRISPR-Cas9 system …
versatile manipulation of a variety of organisms and adaptation of the CRISPR-Cas9 system …
Genome editing in Ustilago maydis using the CRISPR–Cas system
M Schuster, G Schweizer, S Reissmann… - Fungal Genetics and …, 2016 - Elsevier
This communication describes the establishment of the type II bacterial CRISPR–Cas9
system to efficiently disrupt target genes in the fungal maize pathogen Ustilago maydis. A …
system to efficiently disrupt target genes in the fungal maize pathogen Ustilago maydis. A …