Polysaccharide-degrading microorganisms express a repertoire of hydrolytic enzymes that
act in synergy on plant cell wall and other natural polysaccharides to elicit the degradation of …

▪ Abstract Protein splicing is a form of posttranslational processing that consists of the
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme
(Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE …

A variety of nuclear magnetic resonance (NMR) applications have been developed for
structure-based drug discovery (SBDD). NMR provides many advantages over other …

A third of the genes in prokaryotic and eukaryotic genomes encode membrane proteins that
are either essential for signal transduction and solute transport or function as scaffold …

▪ Abstract Inteins are genetic elements that disrupt the coding sequence of genes. However,
in contrast to introns, inteins are transcribed and translated together with their host protein …

Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this
process known as protein splicing, the intein itself is not present in the final sequence, thus …

Transverse relaxation-optimized spectroscopy (TROSY), in combination with various isotope-
labeling techniques, has opened avenues to study biomolecules with molecular masses of …

The development of novel isotope labeling strategies for proteins has facilitated the study of
the structure and dynamics of these molecules. In addition, the recent emergence of …

New strategies and technical advances in NMR spectroscopy and biochemical methods for
isotope labeling have enabled solution NMR studies of biomacromolecular systems of 100 …