In Silico Constraint-Based Strain Optimization Methods: the Quest for Optimal Cell Factories

P Maia, M Rocha, I Rocha - Microbiology and Molecular Biology …, 2016 - Am Soc Microbiol
Shifting from chemical to biotechnological processes is one of the cornerstones of 21st
century industry. The production of a great range of chemicals via biotechnological means is …

Elementary flux modes in a nutshell: properties, calculation and applications

J Zanghellini, DE Ruckerbauer, M Hanscho… - Biotechnology …, 2013 - Wiley Online Library
Elementary flux mode (EFM) analysis allows the unbiased decomposition of a metabolic
network into minimal functional units, making it a powerful tool for metabolic engineering …

Elucidating plant-microbe-environment interactions through omics-enabled metabolic modelling using synthetic communities

AE Beck, M Kleiner, AK Garrell - Frontiers in Plant Science, 2022 - frontiersin.org
With a growing world population and increasing frequency of climate disturbance events, we
are in dire need of methods to improve plant productivity, resilience, and resistance to both …

Metabolomics integrated elementary flux mode analysis in large metabolic networks

MP Gerstl, DE Ruckerbauer, D Mattanovich… - Scientific reports, 2015 - nature.com
Elementary flux modes (EFMs) are non-decomposable steady-state pathways in metabolic
networks. They characterize phenotypes, quantify robustness or identify engineering targets …

Complete enumeration of elementary flux modes through scalable demand-based subnetwork definition

KA Hunt, JP Folsom, RL Taffs, RP Carlson - Bioinformatics, 2014 - academic.oup.com
Motivation: Elementary flux mode analysis (EFMA) decomposes complex metabolic network
models into tractable biochemical pathways, which have been used for rational design and …

Elementary vectors and autocatalytic sets for resource allocation in next-generation models of cellular growth

S Müller, D Széliová, J Zanghellini - PLoS computational biology, 2022 - journals.plos.org
Traditional (genome-scale) metabolic models of cellular growth involve an approximate
biomass “reaction”, which specifies biomass composition in terms of precursor metabolites …

tEFMA: computing thermodynamically feasible elementary flux modes in metabolic networks

MP Gerstl, C Jungreuthmayer, J Zanghellini - Bioinformatics, 2015 - academic.oup.com
Elementary flux modes (EFMs) are important structural tools for the analysis of metabolic
networks. It is known that many topologically feasible EFMs are biologically irrelevant …

Logic programming-based Minimal Cut Sets reveal consortium-level therapeutic targets for chronic wound infections

M Mahout, RP Carlson, L Simon, S Peres - npj Systems Biology and …, 2024 - nature.com
Abstract Minimal Cut Sets (MCSs) identify sets of reactions which, when removed from a
metabolic network, disable certain cellular functions. The traditional search for MCSs within …

Green pathways: metabolic network analysis of plant systems

LM Dersch, V Beckers, C Wittmann - Metabolic engineering, 2016 - Elsevier
Metabolic engineering of plants with enhanced crop yield and value-added compositional
traits is particularly challenging as they probably exhibit the highest metabolic network …

Minimizing enzyme mass to decompose flux distribution for identifying biologically relevant elementary flux modes

J Chen, Y Huang, C Zhong - Biosystems, 2023 - Elsevier
The flux distribution in metabolic network can be decomposed as non-negative linear
combinations of elementary flux modes (EFMs). Identifying biologically relevant EFM …