A comprehensive guide to pilus biogenesis in Gram-negative bacteria

MK Hospenthal, TRD Costa, G Waksman - Nature reviews microbiology, 2017 - nature.com
Pili are crucial virulence factors for many Gram-negative pathogens. These surface
structures provide bacteria with a link to their external environments by enabling them to …

Application of hyperthermophiles in sludge composting: a review

S Chen, J Gu, S Zhang, R Yu - Environmental Chemistry Letters, 2024 - Springer
Sewage sludge treatment and disposal remains a challenging problem. Composting of
sewage sludges is gaining increasing attention due to its cost-effectiveness, operation …

Structure of a type IV pilus machinery in the open and closed state

VAM Gold, R Salzer, B Averhoff, W Kühlbrandt - Elife, 2015 - elifesciences.org
Proteins of the secretin family form large macromolecular complexes, which assemble in the
outer membrane of Gram-negative bacteria. Secretins are major components of type II and III …

From metagenomics to pure culture: isolation and characterization of the moderately halophilic bacterium Spiribacter salinus gen. nov., sp. nov

MJ León, AB Fernández, R Ghai… - Applied and …, 2014 - Am Soc Microbiol
Recent metagenomic studies on saltern ponds with intermediate salinities have determined
that their microbial communities are dominated by both Euryarchaeota and halophilic …

Binding to pyruvylated compounds as an ancestral mechanism to anchor the outer envelope in primitive bacteria

F Cava, MA De Pedro, H Schwarz… - Molecular …, 2004 - Wiley Online Library
Electron microscopy of isolated cell walls of the ancient bacterium Thermus thermophilus
revealed that most of the peptidoglycan (PG) surface, apart from the septal region, was …

Expression and use of superfolder green fluorescent protein at high temperatures in vivo: a tool to study extreme thermophile biology

F Cava, MA De Pedro, E Blas‐Galindo… - Environmental …, 2008 - Wiley Online Library
Superfolder GFP (sGFP) is a variant of the Green Fluorescent Protein that folds efficiently
when fused to poorly folded proteins. In this study, we show that sGFP, but not enhanced …

Development of a gene expression vector for Thermus thermophilus based on the promoter of the respiratory nitrate reductase

R Moreno, O Zafra, F Cava, J Berenguer - Plasmid, 2003 - Elsevier
A specific expression system for Thermus spp. is described. Plasmid pMKE1 contains
replicative origins for Escherichia coli and Thermus spp., a selection gene encoding a …

The Thermus thermophiluscomEA/comEC operon is associated with DNA binding and regulation of the DNA translocator and type IV pili

R Salzer, T Kern, F Joos… - Environmental …, 2016 - Wiley Online Library
Natural transformation systems and type IV pili are linked in many naturally competent
bacteria. In the G ram‐negative bacterium T hermus thermophilus, a leading model …

The role of single subunits of the DNA transport machinery of Thermus thermophilus HB27 in DNA binding and transport

C Schwarzenlander, W Haase… - Environmental …, 2009 - Wiley Online Library
Thermus thermophilus HB27 is well known for its extraordinary trait of high frequencies of
natural transformation, which is considered a major mechanism of horizontal gene transfer …

The role of the nitrate respiration element of Thermus thermophilus in the control and activity of the denitrification apparatus

F Cava, O Zafra, MS Da Costa… - Environmental …, 2008 - Wiley Online Library
The nitrate conjugative element (NCE) encodes the ability to respire nitrate in the facultative
Thermus thermophilus NAR1 strain. This process is carried out by two heterotetrameric …