mScarlet3: a brilliant and fast-maturing red fluorescent protein

TWJ Gadella Jr, L van Weeren, J Stouthamer… - Nature …, 2023 - nature.com
We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with
fast and complete maturation, as well as record brightness, quantum yield (75%) and …

[HTML][HTML] Structures of liganded glycosylphosphatidylinositol transamidase illuminate GPI-AP biogenesis

Y Xu, T Li, Z Zhou, J Hong, Y Chao, Z Zhu… - Nature …, 2023 - nature.com
Many eukaryotic receptors and enzymes rely on glycosylphosphatidylinositol (GPI) anchors
for membrane localization and function. The transmembrane complex GPI-T recognizes …

Targeting ultrafast spectroscopic insights into red fluorescent proteins

TD Krueger, C Chen, C Fang - Chemistry–An Asian Journal, 2023 - Wiley Online Library
Red fluorescent proteins (RFPs) represent an increasingly popular class of genetically
encodable bioprobes and biomarkers that can advance next‐generation breakthroughs …

Functional principles of genetically encoded fluorescent biosensors for metabolism and their quantitative use

D Koveal - Journal of Neurochemistry, 2024 - Wiley Online Library
Genetically encoded fluorescent biosensors provide an attractive means of measuring
chemical changes in single cells on fast timescales (milliseconds to seconds). While their …

[HTML][HTML] Structural characterization of fluorescent proteins using tunable femtosecond stimulated Raman spectroscopy

C Chen, JN Henderson, DA Ruchkin, JM Kirsh… - International journal of …, 2023 - mdpi.com
The versatile functions of fluorescent proteins (FPs) as fluorescence biomarkers depend on
their intrinsic chromophores interacting with the protein environment. Besides X-ray …

[HTML][HTML] Optical, photo-physical and photo-stability characterizations of malachite green as a laser dye, combined with TD-DFT simulations

AR Ghazy, DF Abotalb, ER Kenawy, R Ghazy… - Optical and Quantum …, 2024 - Springer
The optical properties of Malachite green (Mg) dye were examined experimentally, including
absorption, optical energy gab, refractive index, dielectric constant, optical conductivity and …

[HTML][HTML] Molecular basis of the inositol deacylase PGAP1 involved in quality control of GPI-AP biogenesis

J Hong, T Li, Y Chao, Y Xu, Z Zhu, Z Zhou, W Gu… - Nature …, 2024 - nature.com
The secretion and quality control of glycosylphosphatidylinositol-anchored proteins (GPI-
APs) necessitates post-attachment remodeling initiated by the evolutionarily conserved …

Influence of fluorescence lifetime selections and conformational flexibility on brightness of fusionRed variants

S Mukherjee, N Douglas, R Jimenez - The Journal of Physical …, 2024 - ACS Publications
Fluorescent proteins (FPs) for bioimaging are typically developed by screening mutant
libraries for clones with improved photophysical properties. This approach has resulted in …

[HTML][HTML] Capturing excited-state structural snapshots of evolutionary green-to-red photochromic fluorescent proteins

TD Krueger, JN Henderson, IL Breen, L Zhu… - Frontiers in …, 2023 - frontiersin.org
Photochromic fluorescent proteins (FPs) have proved to be indispensable luminous probes
for sophisticated and advanced bioimaging techniques. Among them, an interplay between …

Molecular dynamics force field parameters for the EGFP chromophore and some of its analogues

KL Breyfogle, DL Blood, AM Rosnik… - The Journal of Physical …, 2023 - ACS Publications
Fluorescent proteins (FPs) have had an enormous impact on molecular and cellular biology
and are employed in a wide range of studies of molecular structure and dynamics. Yet, only …