mScarlet3: a brilliant and fast-maturing red fluorescent protein
TWJ Gadella Jr, L van Weeren, J Stouthamer… - Nature …, 2023 - nature.com
We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with
fast and complete maturation, as well as record brightness, quantum yield (75%) and …
fast and complete maturation, as well as record brightness, quantum yield (75%) and …
[HTML][HTML] Structures of liganded glycosylphosphatidylinositol transamidase illuminate GPI-AP biogenesis
Y Xu, T Li, Z Zhou, J Hong, Y Chao, Z Zhu… - Nature …, 2023 - nature.com
Many eukaryotic receptors and enzymes rely on glycosylphosphatidylinositol (GPI) anchors
for membrane localization and function. The transmembrane complex GPI-T recognizes …
for membrane localization and function. The transmembrane complex GPI-T recognizes …
Targeting ultrafast spectroscopic insights into red fluorescent proteins
Red fluorescent proteins (RFPs) represent an increasingly popular class of genetically
encodable bioprobes and biomarkers that can advance next‐generation breakthroughs …
encodable bioprobes and biomarkers that can advance next‐generation breakthroughs …
Functional principles of genetically encoded fluorescent biosensors for metabolism and their quantitative use
D Koveal - Journal of Neurochemistry, 2024 - Wiley Online Library
Genetically encoded fluorescent biosensors provide an attractive means of measuring
chemical changes in single cells on fast timescales (milliseconds to seconds). While their …
chemical changes in single cells on fast timescales (milliseconds to seconds). While their …
[HTML][HTML] Structural characterization of fluorescent proteins using tunable femtosecond stimulated Raman spectroscopy
The versatile functions of fluorescent proteins (FPs) as fluorescence biomarkers depend on
their intrinsic chromophores interacting with the protein environment. Besides X-ray …
their intrinsic chromophores interacting with the protein environment. Besides X-ray …
[HTML][HTML] Optical, photo-physical and photo-stability characterizations of malachite green as a laser dye, combined with TD-DFT simulations
The optical properties of Malachite green (Mg) dye were examined experimentally, including
absorption, optical energy gab, refractive index, dielectric constant, optical conductivity and …
absorption, optical energy gab, refractive index, dielectric constant, optical conductivity and …
[HTML][HTML] Molecular basis of the inositol deacylase PGAP1 involved in quality control of GPI-AP biogenesis
J Hong, T Li, Y Chao, Y Xu, Z Zhu, Z Zhou, W Gu… - Nature …, 2024 - nature.com
The secretion and quality control of glycosylphosphatidylinositol-anchored proteins (GPI-
APs) necessitates post-attachment remodeling initiated by the evolutionarily conserved …
APs) necessitates post-attachment remodeling initiated by the evolutionarily conserved …
Influence of fluorescence lifetime selections and conformational flexibility on brightness of fusionRed variants
S Mukherjee, N Douglas, R Jimenez - The Journal of Physical …, 2024 - ACS Publications
Fluorescent proteins (FPs) for bioimaging are typically developed by screening mutant
libraries for clones with improved photophysical properties. This approach has resulted in …
libraries for clones with improved photophysical properties. This approach has resulted in …
[HTML][HTML] Capturing excited-state structural snapshots of evolutionary green-to-red photochromic fluorescent proteins
TD Krueger, JN Henderson, IL Breen, L Zhu… - Frontiers in …, 2023 - frontiersin.org
Photochromic fluorescent proteins (FPs) have proved to be indispensable luminous probes
for sophisticated and advanced bioimaging techniques. Among them, an interplay between …
for sophisticated and advanced bioimaging techniques. Among them, an interplay between …
Molecular dynamics force field parameters for the EGFP chromophore and some of its analogues
KL Breyfogle, DL Blood, AM Rosnik… - The Journal of Physical …, 2023 - ACS Publications
Fluorescent proteins (FPs) have had an enormous impact on molecular and cellular biology
and are employed in a wide range of studies of molecular structure and dynamics. Yet, only …
and are employed in a wide range of studies of molecular structure and dynamics. Yet, only …