[HTML][HTML] Hydrogels as drug delivery systems: A review of current characterization and evaluation techniques

M Vigata, C Meinert, DW Hutmacher, N Bock - Pharmaceutics, 2020 - mdpi.com
Owing to their tunable properties, controllable degradation, and ability to protect labile
drugs, hydrogels are increasingly investigated as local drug delivery systems. However, a …

[HTML][HTML] Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications

J Sankaran, T Wohland - Communications Biology, 2023 - nature.com
Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …

[图书][B] Principles of fluorescence spectroscopy

JR Lakowicz - 2006 - Springer
The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

Fluorescence correlation spectroscopy: the technique and its applications

O Krichevsky, G Bonnet - Reports on Progress in Physics, 2002 - iopscience.iop.org
Fluorescence correlation spectroscopy (FCS) is an experimental technique using statistical
analysis of the fluctuations of fluorescence in a system in order to decipher dynamic …

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

Z Farka, MJ Mickert, M Pastucha… - Angewandte Chemie …, 2020 - Wiley Online Library
The ability to detect low concentrations of analytes and in particular low‐abundance
biomarkers is of fundamental importance, eg, for early‐stage disease diagnosis. The …

Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy

J Korlach, P Schwille, WW Webb… - Proceedings of the …, 1999 - National Acad Sciences
We report the application of confocal imaging and fluorescence correlation spectroscopy
(FCS) to characterize chemically well-defined lipid bilayer models for biomembranes. Giant …

Biological and chemical applications of fluorescence correlation spectroscopy: a review

ST Hess, S Huang, AA Heikal, WW Webb - Biochemistry, 2002 - ACS Publications
The mathematical concept of fluorescence correlation spectroscopy (FCS) 1 (1) emerged
from quasi-elastic light scattering (QELS) spectroscopy (2) in the early 1970s. Compared to …

Single-vesicle assays using liposomes and cell-derived vesicles: from modeling complex membrane processes to synthetic biology and biomedical applications

H Pick, AC Alves, H Vogel - Chemical reviews, 2018 - ACS Publications
The plasma membrane is of central importance for defining the closed volume of cells in
contradistinction to the extracellular environment. The plasma membrane not only serves as …

[HTML][HTML] Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

R Macháň, M Hof - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2010 - Elsevier
Investigation of lipid lateral mobility in biological membranes and their artificial models
provides information on membrane dynamics and structure; methods based on optical …

[HTML][HTML] Focal volume optics and experimental artifacts in confocal fluorescence correlation spectroscopy

ST Hess, WW Webb - Biophysical journal, 2002 - cell.com
Fluorescence correlation spectroscopy (FCS) can provide a wealth of information about
biological and chemical systems on a broad range of time scales (< 1μs to> 1s). Numerical …