Delivery technologies for genome editing
With the recent development of CRISPR technology, it is becoming increasingly easy to
engineer the genome. Genome-editing systems based on CRISPR, as well as transcription …
engineer the genome. Genome-editing systems based on CRISPR, as well as transcription …
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …
comprise a powerful class of tools that are redefining the boundaries of biological research …
[PDF][PDF] Targeted A-to-G base editing in human mitochondrial DNA with programmable deaminases
Mitochondrial DNA (mtDNA) editing paves the way for disease modeling of mitochondrial
genetic disorders in cell lines and animals and also for the treatment of these diseases in the …
genetic disorders in cell lines and animals and also for the treatment of these diseases in the …
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
Although RNA-guided genome editing via the CRISPR-Cas9 system is now widely used in
biomedical research, genome-wide target specificities of Cas9 nucleases remain …
biomedical research, genome-wide target specificities of Cas9 nucleases remain …
Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system
is an adaptive immune response in prokaryotes, protecting host cells against invading …
is an adaptive immune response in prokaryotes, protecting host cells against invading …
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune
system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas …
system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas …
Re-evaluation of the roles of DROSHA, Exportin 5, and DICER in microRNA biogenesis
Biogenesis of canonical microRNAs (miRNAs) involves multiple steps: nuclear processing of
primary miRNA (pri-miRNA) by DROSHA, nuclear export of precursor miRNA (pre-miRNA) …
primary miRNA (pri-miRNA) by DROSHA, nuclear export of precursor miRNA (pre-miRNA) …
Mitochondrial DNA editing in mice with DddA-TALE fusion deaminases
DddA-derived cytosine base editors (DdCBEs), composed of the split interbacterial toxin
DddAtox, transcription activator-like effector (TALE), and uracil glycosylase inhibitor (UGI) …
DddAtox, transcription activator-like effector (TALE), and uracil glycosylase inhibitor (UGI) …
A guide to genome engineering with programmable nucleases
H Kim, JS Kim - Nature Reviews Genetics, 2014 - nature.com
Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …
Precision mitochondrial DNA editing with high-fidelity DddA-derived base editors
Bacterial toxin DddA-derived cytosine base editors (DdCBEs)—composed of split DddAtox
(a cytosine deaminase specific to double-stranded DNA), custom-designed TALE …
(a cytosine deaminase specific to double-stranded DNA), custom-designed TALE …