Bacterial 1, 3-1, 4-β-glucanases: structure, function and protein engineering

A Planas - Biochimica et Biophysica Acta (BBA)-Protein Structure …, 2000 - Elsevier
1, 3-1, 4-β-Glucanases (or lichenases, EC 3.2. 1.73) hydrolyse linear β-glucans containing β-
1, 3 and β-1, 4 linkages such as cereal β-glucans and lichenan, with a strict cleavage …

Circular permutation of polypeptide chains: implications for protein folding and stability

U Heinemann, M Hahn - Progress in biophysics and molecular biology, 1995 - Elsevier
DNA, RNA and proteins have in common a linear arrangement of their building blocks,
deoxyribonucleotides, ribonucleotides and amino acids, in chains. In most cases, this …

Molecular and Biochemical Characterization of an Endo-β-1, 3-glucanase of the Hyperthermophilic ArchaeonPyrococcus furiosus

Y Gueguen, WGB Voorhorst, J van der Oost… - Journal of Biological …, 1997 - ASBMB
We report here the first molecular characterization of an endo-β-1, 3-glucanase from an
archaeon. Pyrococcus furiosus is a hyperthermophilic archaeon that is capable of …

Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo …

MJ Gosalbes, JA Pérez-González… - Journal of …, 1991 - Am Soc Microbiol
Two genes, xynD and gluB, encoding a xylanase and an endo-beta-(1, 3)-(1, 4)-glucanase
(lichenase) from Bacillus polymyxa have been cloned and expressed in Escherichia coli and …

Enzymatic properties, crystallization, and deduced amino acid sequence of an alkaline endoglucanase from Bacillus circulans

Y Hakamada, K Endo, S Takizawa, T Kobayashi… - … et Biophysica Acta (BBA …, 2002 - Elsevier
A high-isoelectric-point (pI), alkaline endo-1, 4-β-glucanase (Egl-257) of Bacillus circulans
KSM-N257 was purified to homogeneity and crystallized. The purified enzyme hydrolyzed …

Identification of active site carboxylic residues in Bacillus licheniformis 1, 3-1, 4-beta-D-glucan 4-glucanohydrolase by site-directed mutagenesis.

M Juncosa, J Pons, T Dot, E Querol, A Planas - Journal of Biological …, 1994 - Elsevier
Active site residues of 1, 3-1, 4-beta-D-glucan 4-glucanohydrolase (EC 3.2. 1.73) from
Bacillus licheniformis have been identified by site-directed mutagenesis. Previous work …

Characterization, cloning and sequencing of a thermostable endo-(1,3–1,4) β-glucanase-encoding gene from an alkalophilic Bacillus brevis

ME Louw, SJ Reid, TG Watson - Applied microbiology and biotechnology, 1993 - Springer
A Bacillus brevis gene coding for an endo-(1, 3–1, 4)-β-glucanase was cloned in
Escherichia coli and sequenced. The open reading frame contains a sequence of 759 …

Crystal structure of Bacillus licheniformis 1,3‐1,4‐β‐d‐glucan 4‐glucanohydrolase at 1.8 Å resolution

M Hahn, J Pons, A Planas, E Querol… - FEBS …, 1995 - Wiley Online Library
The crystal structure of the 1, 3‐1, 4‐β‐d‐glucan 4‐glucanohydrolase from Bacillus
licheniformis is solved at a resolution of 1.8 Å and refined to R= 16.5%. The protein has a …

Hybrid Bacillus (1-3,1-4)-β-glucanases: engineering thermostable enzymes by construction of hybrid genes

O Olsen, R Borriss, O Simon, KK Thomsen - Molecular and General …, 1991 - Springer
Summary Hybrid (1-3, 1-4)-β-glucanase genes were constructed by extension of
overlapping segments of the (1-3, 1-4)-β-glucanase genes from Bacillus amyloliquefaciens …

A novel β-glucanase gene from Bacillus halodurans C-125

M Akita, K Kayatama, Y Hatada, S Ito… - FEMS microbiology …, 2005 - academic.oup.com
Abstract A novel endo-β-1, 3 (4)-d-glucanase gene was found in the complete genome
sequence of Bacillus halodurans C-125. The gene was previously annotated as an …