[HTML][HTML] Biocatalysis for biorefineries: The case of dye-decolorizing peroxidases

D Silva, CF Rodrigues, C Lorena, PT Borges… - Biotechnology …, 2023 - Elsevier
Abstract Dye-decolorizing Peroxidases (DyPs) are heme-containing enzymes in fungi and
bacteria that catalyze the reduction of hydrogen peroxide to water with concomitant …

Aspartate or arginine? Validated redox state X-ray structures elucidate mechanistic subtleties of FeIV = O formation in bacterial dye-decolorizing peroxidases

M Lučić, MT Wilson, DA Svistunenko, RL Owen… - JBIC Journal of …, 2021 - Springer
Abstract Structure determination of proteins and enzymes by X-ray crystallography remains
the most widely used approach to complement functional and mechanistic studies …

X-ray–induced photoreduction of heme metal centers rapidly induces active-site perturbations in a protein-independent manner

V Pfanzagl, JH Beale, H Michlits, D Schmidt… - Journal of Biological …, 2020 - ASBMB
Since the advent of protein crystallography, atomic-level macromolecular structures have
provided a basis to understand biological function. Enzymologists use detailed structural …

Oxidoreductase enzymes: Characteristics, applications, and challenges as a biocatalyst

Y Cárdenas‐Moreno… - Biotechnology and …, 2023 - Wiley Online Library
Oxidoreductases are enzymes with distinctive characteristics that favor their use in different
areas, such as agriculture, environmental management, medicine, and analytical chemistry …

Comparing ligninolytic capabilities of bacterial and fungal dye-decolorizing peroxidases and class-II peroxidase-catalases

D Linde, I Ayuso-Fernández, M Laloux… - International journal of …, 2021 - mdpi.com
We aim to clarify the ligninolytic capabilities of dye-decolorizing peroxidases (DyPs) from
bacteria and fungi, compared to fungal lignin peroxidase (LiP) and versatile peroxidase …

The enthalpic and entropic terms of the reduction potential of metalloproteins: Determinants and interplay

G Di Rocco, G Battistuzzi, M Borsari… - Coordination Chemistry …, 2021 - Elsevier
Splitting the reduction potential of electron transport (ET) proteins and redox
metalloenzymes into the enthalpic and entropic contributions is an insightful practice to …

Biochemical and molecular characterization of a new heme peroxidase from Aspergillus niger CTM10002, and its application in textile reactive dye decolorization

S Hamdi, F Allala, S Mechri, K Bouacem, H Rekik… - Process …, 2022 - Elsevier
The presence of textile dyes in wastewater of textile factories represents a major
environmental problem, threatening aquatic life. The decolorisation and degradation of …

On the track of long-range electron transfer in b-type dye-decolorizing peroxidases: Identification of a tyrosyl radical by computational prediction and electron …

K Nys, PG Furtmüller, C Obinger, S Van Doorslaer… - Biochemistry, 2021 - ACS Publications
The catalytic activity of dye-decolorizing peroxidases (DyPs) toward bulky substrates,
including anthraquinone dyes, phenolic lignin model compounds, or 2, 2′-azino-bis (3 …

Direct electrochemical generation of catalytically competent oxyferryl species of classes I and P dye decolorizing peroxidases

MF Scocozza, LO Martins, DH Murgida - International Journal of …, 2021 - mdpi.com
This work introduces a novel way to obtain catalytically competent oxyferryl species for two
different dye-decolorizing peroxidases (DyPs) in the absence of H2O2 or any other peroxide …

Biochemical, Biophysical, and Structural Analysis of an Unusual DyP from the Extremophile Deinococcus radiodurans

K Frade, CM Silveira, BA Salgueiro, S Mendes… - Molecules, 2024 - mdpi.com
Dye-decolorizing peroxidases (DyPs) are heme proteins with distinct structural properties
and substrate specificities compared to classical peroxidases. Here, we demonstrate that …