Organoid and spheroid tumor models: techniques and applications
S Gunti, ATK Hoke, KP Vu, NR London Jr - Cancers, 2021 - mdpi.com
Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and
spheroids are different 3D cell culture models that can be cultured with different techniques …
spheroids are different 3D cell culture models that can be cultured with different techniques …
Modeling neoplastic disease with spheroids and organoids
Cancer is a complex disease in which both genetic defects and microenvironmental
components contribute to the development, progression, and metastasization of disease …
components contribute to the development, progression, and metastasization of disease …
Three-dimensional culture systems in cancer research: Focus on tumor spheroid model
Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically
relevant cell–cell and cell–matrix interactions, gene expression and signaling pathway …
relevant cell–cell and cell–matrix interactions, gene expression and signaling pathway …
Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling
Highlights•Traditional drug screening model systems are inadequate.•Organoid-on-a-chip
platforms stand to significantly advance drug and toxicology screening.•A wide variety or …
platforms stand to significantly advance drug and toxicology screening.•A wide variety or …
Three-dimensional cell culture: the missing link in drug discovery
S Breslin, L O'Driscoll - Drug discovery today, 2013 - Elsevier
Cells, grown as monolayers (2D models), are routinely used as initial model systems for
evaluating the effectiveness and safety of libraries of molecules with potential as therapeutic …
evaluating the effectiveness and safety of libraries of molecules with potential as therapeutic …
Drug compound screening in single and integrated multi-organoid body-on-a-chip systems
Current practices in drug development have led to therapeutic compounds being approved
for widespread use in humans, only to be later withdrawn due to unanticipated toxicity …
for widespread use in humans, only to be later withdrawn due to unanticipated toxicity …
Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform
Many drugs have progressed through preclinical and clinical trials and have been available–
for years in some cases–before being recalled by the FDA for unanticipated toxicity in …
for years in some cases–before being recalled by the FDA for unanticipated toxicity in …
Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy
Multicellular spheroids are three dimensional in vitro microscale tissue analogs. The current
article examines the suitability of spheroids as an in vitro platform for testing drug delivery …
article examines the suitability of spheroids as an in vitro platform for testing drug delivery …
Matrigel: from discovery and ECM mimicry to assays and models for cancer research
G Benton, I Arnaoutova, J George, HK Kleinman… - Advanced drug delivery …, 2014 - Elsevier
The basement membrane is an important extracellular matrix that is found in all epithelial
and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to …
and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to …
Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation
M Vinci, S Gowan, F Boxall, L Patterson… - BMC biology, 2012 - Springer
Background There is overwhelming evidence that in vitro three-dimensional tumor cell
cultures more accurately reflect the complex in vivo microenvironment than simple two …
cultures more accurately reflect the complex in vivo microenvironment than simple two …