Small, but powerful and attractive: 19F in biomolecular NMR

AM Gronenborn - Structure, 2022 - cell.com
Nuclear magnetic resonance (NMR) spectroscopy is a versatile tool for probing structure,
dynamics, folding, and interactions at atomic resolution. While naturally occurring …

Advances in the study of GPCRs by 19F NMR

LP Picard, RS Prosser - Current Opinion in Structural Biology, 2021 - Elsevier
Crystallography and cryo-electron microscopy have advanced atomic resolution
perspectives of inactive and active states of G protein-coupled receptors (GPCRs), alone …

Visualizing Proteins in Mammalian Cells by 19F NMR Spectroscopy

W Zhu, AJ Guseman, F Bhinderwala… - Angewandte Chemie …, 2022 - Wiley Online Library
In‐cell NMR spectroscopy is a powerful tool to investigate protein behavior in physiologically
relevant environments. Although proven valuable for disordered proteins, we show that in …

Multi‐Site Conformational Exchange in the Synthetic Neomycin‐Sensing Riboswitch Studied by 19F NMR

JH Overbeck, J Vögele, F Nussbaumer… - Angewandte Chemie …, 2023 - Wiley Online Library
The synthetic neomycin‐sensing riboswitch interacts with its cognate ligand neomycin as
well as with the related antibiotics ribostamycin and paromomycin. Binding of these …

Application of W-band 19 F electron nuclear double resonance (ENDOR) spectroscopy to distance measurement using a trityl spin probe and a fluorine label

NB Asanbaeva, AA Sukhanov, AA Diveikina… - Physical Chemistry …, 2022 - pubs.rsc.org
Recently, Marina Bennati and coworkers (M. Bennati et al., Angew. Chem., Int. Ed., 2020, 59,
373–379., M. Bennati et al., J. Magn. Reson., 2021, 333, 107091) proposed to use electron …

Large domain movements through the lipid bilayer mediate substrate release and inhibition of glutamate transporters

X Wang, O Boudker - Elife, 2020 - elifesciences.org
Glutamate transporters are essential players in glutamatergic neurotransmission in the
brain, where they maintain extracellular glutamate below cytotoxic levels and allow for …

Millisecond dynamics of an unlabeled amino acid transporter

TR Matin, GR Heath, GHM Huysmans… - Nature …, 2020 - nature.com
Excitatory amino acid transporters (EAATs) are important in many physiological processes
and crucial for the removal of excitatory amino acids from the synaptic cleft. Here, we …

Environmentally Ultrasensitive Fluorine Probe to Resolve Protein Conformational Ensembles by 19F NMR and Cryo-EM

Y Huang, KD Reddy, C Bracken, B Qiu… - Journal of the …, 2023 - ACS Publications
Limited chemical shift dispersion represents a significant barrier to studying multistate
equilibria of large membrane proteins by 19F NMR. We describe a novel monofluoroethyl …

Solution NMR investigations of integral membrane proteins: Challenges and innovations

M Necelis, C McDermott, MB Dufrisne… - Current Opinion in …, 2023 - Elsevier
Compared to soluble protein counterparts, the understanding of membrane protein stability,
solvent interactions, and function are not as well understood. Recent advancements in …

Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy

C Le Bon, B Michon, JL Popot… - Quarterly Reviews of …, 2021 - cambridge.org
Over the past decade, the structural biology of membrane proteins (MPs) has taken a new
turn thanks to epoch-making technical progress in single-particle electron cryo-microscopy …