Past, present and future of ICSI in livestock species

O Briski, DF Salamone - Animal Reproduction Science, 2022 - Elsevier
During the past 2 decades, intracytoplasmic sperm injection (ICSI) has become a routine
technique for clinical applications in humans. The widespread use among domestic species …

Intracytoplasmic sperm injection in domestic and wild mammals

DF Salamone, NG Canel, MB Rodríguez - Reproduction, 2017 - rep.bioscientifica.com
Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical
applications in the horse-breeding industry. However, both ICSI blastocyst and offspring …

Bovine ICSI: Limiting factors, strategies to improve its efficiency and alternative approaches

F Fuentes, E Munoz, MJ Contreras, ME Arias, R Felmer - Zygote, 2022 - cambridge.org
Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technique mainly used to
overcome severe infertility problems associated with the male factor, but in cattle its …

Intracytoplasmic sperm injection in cattle

V Unnikrishnan, J Kastelic, J Thundathil - Genes, 2021 - mdpi.com
Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured
oocyte. Although this reproductive technology is successfully used in humans and many …

Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals

P Bosch, DO Forcato, FE Alustiza, AP Alessio… - Cellular and Molecular …, 2015 - Springer
Transgenic farm animals are attractive alternative mammalian models to rodents for the
study of developmental, genetic, reproductive and disease-related biological questions, as …

Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas

DK Bellido-Quispe, FRM Lengua, MC Huamani… - Animal reproduction …, 2024 - Elsevier
Low motility and low sperm concentration are characteristics of alpaca semen. Thus, the
intracytoplasmic sperm injection (ICSI) technique represents an alternative to improve the …

Production of transgenic livestock: overview of transgenic technologies

G Laible - Animal Biotechnology 2: Emerging Breeding …, 2018 - Springer
Transgenic livestock came into existence in 1985 with the production of the first transgenic
pigs and sheep (Hammer et al., 1985). Since then, various technologies have been …

Efficiency of sperm-mediated gene transfer in the ovine by laparoscopic insemination, in vitro fertilization and ICSI

F Pereyra-Bonnet, A Gibbons, M Cueto… - … of Reproduction and …, 2011 - jstage.jst.go.jp
Transgenesis constitutes an important tool for pharmacological protein production and
livestock improvement. We evaluated the potential of laparoscopic insemination (LI), in vitro …

DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality

AE Ynsaurralde-Rivolta, M Suvá, CG Luchetti… - Theriogenology, 2020 - Elsevier
The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant
property was poorly explored. In this study, we evaluated the effect of DMSO …

Improved bovine ICSI outcomes by sperm selected after combined heparin-glutathione treatment

MH Sekhavati, F Shadanloo, MS Hosseini… - … " Cloning and Stem …, 2012 - liebertpub.com
Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted
reproductive techniques (ART), the efficiency of this method is still far from satisfactory in …