Food analysis and control are crucial aspects in food research and production in order to
ensure quality and safety of food products. Electrochemical biosensors based on enzymes …

Highlights•Protein engineering has been widely used to improve biosensor performance.•
Enzymatic catalysis and electron transfer both play essential roles.•Oriented enzyme …

The electrochemical enzyme sensors based on direct electron transfer (DET)-type
oxidoreductase-based enzymes are ideal for continuous and in vivo monitoring. However …

Direct bioelectrocatalysis applied in biosensors, biofuel cells, and bioelectrosynthesis is
based on an efficient electron transfer between enzymes and electrodes in the absence of …

Cytochromes are a large and well-known group of electron transfer proteins, but only very
few are used in bioelectrochemistry, eg to serve as electron shuttles between biocatalysts …

Among the various types of enzyme-based biosensors, sensors utilizing enzymes capable of
direct electron transfer (DET) are recognized as the most ideal. However, only a limited …

Controlling the orientation of redox enzymes on electrode surfaces is essential in the
development of direct electron transfer (DET)-based bioelectrocatalytic systems. The …

Advances in protein engineering-enabled enzyme immobilization technologies have
significantly improved enzyme–electrode wiring in enzymatic electrochemical systems …

Biosensors have garnered substantial attention as an emerging toolbox for analyzing
biomarkers, physiological processes, food, and environmental metrices. Rapid, sensitive …

A glucose biosensor was layer-by-layer assembled on a modified glassy carbon electrode
(GCE) from a nanocomposite of NAD (P)+-dependent glucose dehydrogenase, aminated …