In a diverse group of organisms that includes Caenorhabditis elegans, Drosophila, planaria,
hydra, trypanosomes, fungi and plants, the introduction of double-stranded RNAs inhibits …

Background: The injection of double-stranded RNA (dsRNA) has been shown to induce a
potent sequence-specific inhibition of gene function in diverse invertebrate and vertebrate …

Insect cells are relatively cheap to maintain and are capable of producing accurately
translated and correctly processed heterologous proteins. Recent research has focused on …

The promoters of heat shock protein genes are among the best-studied inducible eucaryotic
promoters. Regions responsible for heat regulation have been identified previously by …

We have adopted Drosophila tissue culture cells as a host system for studying the structure
and function of mammalian transcription factors. These cells provide an Spl-deficient …

In vertebrates, many cytokines and growth factors have been identified as activators of the
JAK/STAT signaling pathway. In Drosophila, JAK and STAT molecules have been isolated …

The Toll receptor was originally identified as an indispensable molecule for Drosophila
embryonic development and subsequently as an essential component of innate immunity …

Publisher Summary This chapter focuses on techniques for transforming Drosophila cell
lines. The chapter shows how individual lines differ substantially in their transfectabilities by …

Wnt/β-catenin signaling is initiated at the cell surface by association of secreted Wnt with its
receptors Frizzled (Fz) and low density lipoprotein receptor-related protein 5/6 (LRP5/6). The …

Sp1, Sp3 (SPR‐2) and Sp4 (SPR‐1) are human sequence‐specific DNA binding proteins
with very similar structural features. In this report, we have analyzed Sp3 in direct …