Intracellular mRNA transport and localized translation

S Das, M Vera, V Gandin, RH Singer… - Nature reviews Molecular …, 2021 - nature.com
Fine-tuning cellular physiology in response to intracellular and environmental cues requires
precise temporal and spatial control of gene expression. High-resolution imaging …

In the right place at the right time: visualizing and understanding mRNA localization

AR Buxbaum, G Haimovich, RH Singer - Nature reviews Molecular cell …, 2015 - nature.com
The spatial regulation of protein translation is an efficient way to create functional and
structural asymmetries in cells. Recent research has furthered our understanding of how …

Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs

X Chen, D Zhang, N Su, B Bao, X Xie, F Zuo… - Nature …, 2019 - nature.com
Abstract Fluorescent RNAs (FRs), aptamers that bind and activate fluorescent dyes, have
been used to image abundant cellular RNA species. However, limitations such as low …

Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs): post-transcriptional drivers of cancer progression?

JL Bell, K Wächter, B Mühleck, N Pazaitis… - Cellular and molecular …, 2013 - Springer
The insulin-like growth factor-2 mRNA-binding proteins 1, 2, and 3 (IGF2BP1, IGF2BP2,
IGF2BP3) belong to a conserved family of RNA-binding, oncofetal proteins. Several studies …

The central dogma decentralized: new perspectives on RNA function and local translation in neurons

CE Holt, EM Schuman - Neuron, 2013 - cell.com
The elaborate morphology of neurons together with the information processing that occurs in
remote dendritic and axonal compartments makes the use of decentralized cell biological …

Keratins are asymmetrically inherited fate determinants in the mammalian embryo

HYG Lim, YD Alvarez, M Gasnier, Y Wang, P Tetlak… - Nature, 2020 - nature.com
To implant in the uterus, the mammalian embryo first specifies two cell lineages: the
pluripotent inner cell mass that forms the fetus, and the outer trophectoderm layer that forms …

Visualization of dynamics of single endogenous mRNA labeled in live mouse

HY Park, H Lim, YJ Yoon, A Follenzi, C Nwokafor… - Science, 2014 - science.org
The transcription and transport of messenger RNA (mRNA) are critical steps in regulating
the spatial and temporal components of gene expression, but it has not been possible to …

Live cell imaging of single RNA molecules with fluorogenic Mango II arrays

AD Cawte, PJ Unrau, DS Rueda - Nature communications, 2020 - nature.com
RNA molecules play vital roles in many cellular processes. Visualising their dynamics in live
cells at single-molecule resolution is essential to elucidate their role in RNA metabolism …

Glutamate-induced RNA localization and translation in neurons

YJ Yoon, B Wu, AR Buxbaum, S Das… - Proceedings of the …, 2016 - National Acad Sciences
Localization of mRNA is required for protein synthesis to occur within discrete intracellular
compartments. Neurons represent an ideal system for studying the precision of mRNA …

Mapping translation'hot-spots' in live cells by tracking single molecules of mRNA and ribosomes

ZB Katz, BP English, T Lionnet, YJ Yoon, N Monnier… - Elife, 2016 - elifesciences.org
Messenger RNA localization is important for cell motility by local protein translation.
However, while single mRNAs can be imaged and their movements tracked in single cells, it …