Paramagnetic chemical probes for studying biological macromolecules

Q Miao, C Nitsche, H Orton, M Overhand… - Chemical …, 2022 - ACS Publications
Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Predicting the structures of glycans, glycoproteins, and their complexes

RJ Woods - Chemical reviews, 2018 - ACS Publications
Complex carbohydrates are ubiquitous in nature, and together with proteins and nucleic
acids they comprise the building blocks of life. But unlike proteins and nucleic acids …

[HTML][HTML] Glycan structures and their interactions with proteins. A NMR view

A Gimeno, P Valverde, A Ardá… - Current opinion in …, 2020 - Elsevier
Highlights•NMR methods are of paramount importance to deduce structure, dynamics and
recognition of complex glycans.•The intrinsic low sensitivity of NMR for studying glycans and …

Novel NMR avenues to explore the conformation and interactions of glycans

P Valverde, JI Quintana, JI Santos, A Ardá… - ACS …, 2019 - ACS Publications
This perspective article is focused on the presentation of the latest advances in NMR
methods and applications that are behind the exciting achievements in the understanding of …

Conformational effects of N-glycan core fucosylation of immunoglobulin G Fc region on its interaction with Fcγ receptor IIIa

Y Sakae, T Satoh, H Yagi, S Yanaka, T Yamaguchi… - Scientific reports, 2017 - nature.com
Antibody-dependent cellular cytotoxicity (ADCC) is promoted through interaction between
the Fc region of immunoglobulin G1 (IgG1) and Fcγ receptor IIIa (FcγRIIIa), depending on N …

Three-dimensional structures of carbohydrates and where to find them

SI Scherbinina, PV Toukach - International journal of molecular sciences, 2020 - mdpi.com
Analysis and systematization of accumulated data on carbohydrate structural diversity is a
subject of great interest for structural glycobiology. Despite being a challenging task …

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

DJ Harvey - Mass Spectrometry Reviews, 2023 - Wiley Online Library
This review is the tenth update of the original article published in 1999 on the application of
matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry …

Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems

S Yanaka, H Yagi, R Yogo, M Yagi-Utsumi… - Journal of Biomolecular …, 2018 - Springer
Glycoproteins are characterized by the heterogeneous and dynamic nature of their glycan
moieties, which hamper crystallographic analysis. NMR spectroscopy provides potential …

Conformational preferences of triantennary and tetraantennary hybrid N-glycans in aqueous solution: Insights from 20 μs long atomistic molecular dynamic …

R Roy, S Poddar, MF Sk, P Kar - Journal of Biomolecular Structure …, 2023 - Taylor & Francis
In the current study, we have investigated the conformational dynamics of a triantennary (N-
glycan1) and tetraantennary (N-glycan2) hybrid N-glycans found on the surface of the HIV …

The flexibility of oligosaccharides unveiled through residual dipolar coupling analysis

A Poveda, G Fittolani, PH Seeberger… - Frontiers in Molecular …, 2021 - frontiersin.org
The intrinsic flexibility of glycans complicates the study of their structures and dynamics,
which are often important for their biological function. NMR has provided insights into the …