The current state of biosensor-based techniques for amplification-free microRNA (miRNA)
detection is critically reviewed. Comparison with non-sensor and amplification-based …

Nucleic acids (DNA and RNA) dynamically fold and unfold to exert their functions in cells.
These folding and unfolding behaviours are also the basis for various technical applications …

Metals are essential in many biological processes, and metal ions are modeled in roughly
40% of the macromolecular structures in the Protein Data Bank (PDB). However, a …

The determination of annealing temperature is a critical step in PCR design. This parameter
is typically derived from the melting temperature of the PCR primers, so for successful PCR …

The DINAMelt web server simulates the melting of one or two single-stranded nucleic acids
in solution. The goal is to predict not just a melting temperature for a hybridized pair of …

DNA and RNA oligomers are used in a myriad of diverse biological and biochemical
experiments. These oligonucleotides are designed to have unique biophysical, chemical …

Accurate predictions of DNA stability in physiological and enzyme buffers are important for
the design of many biological and biochemical assays. We therefore investigated the effects …

A new 3-Site-Per-Nucleotide coarse-grained model for DNA is presented. The model
includes anisotropic potentials between bases involved in base stacking and base pair …

Metal ions play crucial roles in thermal stability and folding kinetics of nucleic acids. For ions
(especially multivalent ions) in the close vicinity of nucleic acid surface, interion correlations …

Locked nucleic acids (LNA) show remarkable affinity and specificity against native DNA
targets. Effects of LNA modifications on mismatch discrimination were studied as a function …