Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Fluorescence lifetime techniques in medical applications
L Marcu - Annals of biomedical engineering, 2012 - Springer
This article presents an overview of time-resolved (lifetime) fluorescence techniques used in
biomedical diagnostics. In particular, we review the development of time-resolved …
biomedical diagnostics. In particular, we review the development of time-resolved …
Separating NADH and NADPH fluorescence in live cells and tissues using FLIM
NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form,
NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced …
NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced …
[图书][B] Advanced time-correlated single photon counting applications
W Becker - 2015 - Springer
Time-correlated single photon counting (TCSPC) was born 54 years ago, in 1960, when
Bollinger and Thomas published a paper on the 'Measurement of Time Dependence of …
Bollinger and Thomas published a paper on the 'Measurement of Time Dependence of …
Intraoperative fluorescence-guided resection of high-grade gliomas: a comparison of the present techniques and evolution of future strategies
Objective Fluorescence guidance has a demonstrated potential in maximizing the extent of
high-grade glioma resection. Different fluorophores (fluorescent biomarkers), including 5 …
high-grade glioma resection. Different fluorophores (fluorescent biomarkers), including 5 …
Intraoperative margin assessment in oral and oropharyngeal cancer using label-free fluorescence lifetime imaging and machine learning
Objective: To demonstrate the diagnostic ability of label-free, point-scanning, fiber-based
Fluorescence Lifetime Imaging (FLIm) as a means of intraoperative guidance during oral …
Fluorescence Lifetime Imaging (FLIm) as a means of intraoperative guidance during oral …
Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery
Y Sun, N Hatami, M Yee, J Phipps… - … of biomedical optics, 2010 - spiedigitallibrary.org
We demonstrate for the first time the application of an endoscopic fluorescence lifetime
imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma …
imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma …
Optical spectroscopy for in vivo medical diagnosis—a review of the state of the art and future perspectives
When light is incident to a biological tissue surface, combinations of optical processes occur,
such as reflection, absorption, elastic and non-elastic scattering, and fluorescence. Analysis …
such as reflection, absorption, elastic and non-elastic scattering, and fluorescence. Analysis …
Optical technologies for intraoperative neurosurgical guidance
Biomedical optics is a broadly interdisciplinary field at the interface of optical engineering,
biophysics, computer science, medicine, biology, and chemistry, helping us understand light …
biophysics, computer science, medicine, biology, and chemistry, helping us understand light …
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low- and high-grade glioma surgery
Biomarkers are indicators of biological processes and hold promise for the diagnosis and
treatment of disease. Gliomas represent a heterogeneous group of brain tumors with marked …
treatment of disease. Gliomas represent a heterogeneous group of brain tumors with marked …