Mixing, aeration and cell damage, 30+ years later: what we learned, how it affected the cell culture industry and what we would like to know more about
JJ Chalmers - Current Opinion in Chemical Engineering, 2015 - Elsevier
Highlights•Animal cell culture producing biopharmaceuticals is a 200 billion dollar
industry.•In thirty years, final product concentrations have risen from 50 mg/L to 25 …
industry.•In thirty years, final product concentrations have risen from 50 mg/L to 25 …
Dynamic Single-Use Bioreactors Used in Modern Liter- and m3- Scale Biotechnological Processes: Engineering Characteristics and Scaling Up
C Löffelholz, SC Kaiser, M Kraume, R Eibl… - Disposable bioreactors II, 2014 - Springer
During the past 10 years, single-use bioreactors have been well accepted in modern
biopharmaceutical production processes targeting high-value products. Up to now, such …
biopharmaceutical production processes targeting high-value products. Up to now, such …
Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors
BS Borys, T Dang, T So, L Rohani, T Revay… - Stem Cell Research & …, 2021 - Springer
Background Human induced pluripotent stem cells (hiPSCs) hold enormous promise in
accelerating breakthroughs in understanding human development, drug screening, disease …
accelerating breakthroughs in understanding human development, drug screening, disease …
Scale‐up of adipose tissue‐derived mesenchymal stem cell production in stirred single‐use bioreactors under low‐serum conditions
C Schirmaier, V Jossen, SC Kaiser… - Engineering in Life …, 2014 - Wiley Online Library
Suspension cultures, in which human mesenchymal stem cells are cultivated on
microcarriers in scalable single‐use stirred bioreactor types, have been shown to be a …
microcarriers in scalable single‐use stirred bioreactor types, have been shown to be a …
CFD based mass transfer modeling of a single use bioreactor for production of monoclonal antibody biotherapeutics
Abstract In this study, Euler-Euler coupled computational fluid dynamics (CFD), species
transport and population balance model (PBM) simulations have been used to model …
transport and population balance model (PBM) simulations have been used to model …
Beyond heuristics: CFD‐based novel multiparameter scale‐up for geometrically disparate bioreactors demonstrated at industrial 2kL–10kL scales
J Scully, LB Considine, MT Smith… - Biotechnology and …, 2020 - Wiley Online Library
The timely delivery of the most up‐to‐date medicines and drug products is essential for
patients throughout the world. Successful scaling of the bioreactors used within the …
patients throughout the world. Successful scaling of the bioreactors used within the …
Production of oncolytic adenovirus and human mesenchymal stem cells in a single‐use, vertical‐wheel bioreactor system: impact of bioreactor design on performance …
MFQ Sousa, MM Silva, D Giroux… - Biotechnology …, 2015 - Wiley Online Library
Anchorage‐dependent cell cultures are used for the production of viruses, viral vectors, and
vaccines, as well as for various cell therapies and tissue engineering applications. Most of …
vaccines, as well as for various cell therapies and tissue engineering applications. Most of …
Improvement of HEK293 cell growth by adapting hydrodynamic stress and predicting cell aggregate size distribution
S Seidel, RW Maschke, F Mozaffari, R Eibl-Schindler… - Bioengineering, 2023 - mdpi.com
HEK293 is a widely used cell line in the fields of research and industry. It is assumed that
these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle …
these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle …
Theoretical and practical issues that are relevant when scaling up hMSC microcarrier production processes
V Jossen, C Schirmer, D Mostafa Sindi… - Stem cells …, 2016 - Wiley Online Library
The potential of human mesenchymal stem cells (hMSCs) for allogeneic cell therapies has
created a large amount of interest. However, this presupposes the availability of efficient …
created a large amount of interest. However, this presupposes the availability of efficient …
Using CFD simulations and statistical analysis to correlate oxygen mass transfer coefficient to both geometrical parameters and operating conditions in a stirred‐tank …
Optimization of a bioreactor design can be an especially challenging process. For instance,
testing different bioreactor vessel geometries and different impeller and sparger types …
testing different bioreactor vessel geometries and different impeller and sparger types …