RNAi efficiency, systemic properties, and novel delivery methods for pest insect control: what we know so far

MR Joga, MJ Zotti, G Smagghe… - Frontiers in physiology, 2016 - frontiersin.org
In recent years, the research on the potential of using RNA interference (RNAi) to suppress
crop pests has made an outstanding growth. However, given the variability of RNAi …

RNA interference in insects: protecting beneficials and controlling pests

E Vogel, D Santos, L Mingels, TW Verdonckt… - Frontiers in …, 2019 - frontiersin.org
Insects constitute the largest and most diverse group of animals on Earth with an equally
diverse virome. The main antiviral immune system of these animals is the post …

Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata, but not in the desert locust …

J Spit, A Philips, N Wynant, D Santos… - Insect biochemistry and …, 2017 - Elsevier
The responsiveness towards orally delivered dsRNA and the potency of a subsequent
environmental RNA interference (RNAi) response strongly differs between different insect …

Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide

S Mehlhorn, VS Hunnekuhl, S Geibel, R Nauen… - Frontiers in …, 2021 - Springer
RNA interference (RNAi) has emerged as a powerful tool for knocking-down gene function
in diverse taxa including arthropods for both basic biological research and application in …

A Systematic Study of RNAi Effects and dsRNA Stability in Tribolium castaneum and Acyrthosiphon pisum, Following Injection and Ingestion of Analogous dsRNAs

M Cao, JA Gatehouse, EC Fitches - International journal of molecular …, 2018 - mdpi.com
RNA interference (RNAi) effects in insects are highly variable and may be largely dependent
upon the stability of introduced double-stranded RNAs to digestion by nucleases. Here, we …

Guanidinium-functionalized interpolyelectrolyte complexes enabling RNAi in resistant insect pests

KH Parsons, MH Mondal, CL McCormick… - …, 2018 - ACS Publications
RNAi-based technologies are ideal for pest control as they can provide species specificity
and spare nontarget organisms. However, in some pests biological barriers prevent use of …

Highly variable dietary RNAi sensitivity among Coleoptera

J Willow, E Veromann - Frontiers in Plant Science, 2021 - frontiersin.org
Many herbivorous beetles (Order Coleoptera) contribute to serious losses in crop yields and
forest trees, and plant biotechnology solutions are being developed with the hope of limiting …

Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis

R Almeida Garcia, L Lima Pepino Macedo… - PloS one, 2017 - journals.plos.org
RNA interference (RNAi) approaches have been applied as a biotechnological tool for
controlling plant insect pests via selective gene down regulation. However, the inefficiency …

RNA interference-mediated knockdown of voltage-gated sodium channel (MpNav) gene causes mortality in peach-potato aphid, Myzus persicae

K Tariq, A Ali, TGE Davies, E Naz, L Naz, S Sohail… - Scientific Reports, 2019 - nature.com
Voltage-gated sodium channels (VGSC) are transmembrane proteins that generate an
action potential in excitable cells and play an essential role in neuronal signaling. Since …

Implementing the sterile insect technique with RNA interference–a review

M Darrington, T Dalmay, NI Morrison… - Entomologia …, 2017 - Wiley Online Library
We review RNA interference (RNA i) of insect pests and its potential for implementing sterile
insect technique (SIT)‐related control. The molecular mechanisms that support RNA i in pest …